Identification of a localized mucosal immune response in rainbow trout, Oncorhynchus mykiss (Walbaum), following immunization with a protein-hapten antigen

Journal of Fish Diseases
C M SwanKenneth D Cain

Abstract

The ability of rainbow trout, Oncorhynchus mykiss (RBT), to produce a localized mucosal immune response was investigated following intraperitoneal (i.p.) or peranal (p.a.) immunization with a protein-hapten carrier, fluorescein isothiocyanate conjugated to keyhole limpet haemocyanin (FITC/KLH). Antibody levels in serum, mucus, tissue culture supernatant from blood and spleen leucocytes, and excised skin, intestine and gill tissues were determined by ELISA. Significantly, elevated antigen-specific antibodies were elicited in both serum and mucus of fish immunized i.p. Mucosal antibody responses, in general, paralleled serum responses over time. Leucocytes isolated from spleen and blood of i.p. immunized fish at week 10 produced significantly elevated antibody levels against FITC when cultured in vitro. Excised skin, intestine and gill tissues from these fish also exhibited significantly elevated antibody responses indicating localized production in the mucosa from tissue-specific B cells. A localized mucosal immune response was elicited only after i.p. and not p.a. immunization, suggesting that systemically stimulated B cells migrate to mucosal tissues where they produce antibodies locally.

References

Jan 1, 1987·Developmental and Comparative Immunology·C J Lobb
Jan 1, 1984·Developmental and Comparative Immunology·E A St Louis-CormierP D Anderson
Jul 1, 1993·Developmental and Comparative Immunology·J H RomboutA J Taverne-Thiele
Jan 1, 1993·Developmental and Comparative Immunology·J H RomboutM I Villena
Jan 1, 1996·Annual Review of Immunology·M R NeutraJ P Kraehenbuhl
Jan 16, 1999·Advances in Veterinary Medicine·S VinitnantharatE Greger
Nov 7, 2001·Developmental and Comparative Immunology·Kenneth D CainRobert L Raison
Mar 13, 2003·Developmental and Comparative Immunology·Takuya IrieHiroshi Kodama
Sep 11, 2003·Clinical and Diagnostic Laboratory Immunology·Joanne L Maki, Harry W Dickerson
Oct 12, 2004·Fish & Shellfish Immunology·Stefaan VervarckeArmand Michoel
Feb 15, 2005·Developmental and Comparative Immunology·Luigi AbelliUmberto Oreste
Apr 7, 2005·Nature Medicine·Jan Holmgren, Cecil Czerkinsky
Sep 27, 2005·Fish & Shellfish Immunology·Allan G Petrie, Anthony E Ellis
Mar 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·David BernardAbdenour Benmansour

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Citations

Nov 13, 2012·PloS One·Louise von Gersdorff JørgensenNiels Lorenzen
Feb 14, 2016·Developmental and Comparative Immunology·Guro Løkka, Erling Olaf Koppang
Dec 5, 2012·Veterinary Immunology and Immunopathology·Victoria A Valdenegro-VegaBarbara F Nowak
Jun 23, 2012·Antiviral Research·Frederick S B KibengeMolly J T Kibenge
Dec 3, 2011·Developmental and Comparative Immunology·Irene SalinasJ Oriol Sunyer
Jan 5, 2011·Fish & Shellfish Immunology·Silvia TorrecillasMarisol Izquierdo
Feb 6, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Shunpei Takiue, Hideo Akiyoshi
Feb 18, 2010·Experimental Dermatology·Sebastian RakersRalf Paus
Oct 9, 2012·Biologicals : Journal of the International Association of Biological Standardization·Maximiliano WildaAlejandra Victoria Capozzo

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