Identification of a major carbohydrate capping group of the L-selectin ligand on high endothelial venules in human lymph nodes as 6-sulfo sialyl Lewis X.

The Journal of Biological Chemistry
C MitsuokaR Kannagi

Abstract

We investigated the molecular species of sulfated sialyl Lewis X determinants, the putative L-selectin ligand, expressed on high endothelial venules (HEV) in human lymph nodes. Comparison of the reactivity pattern of HEV with the reactivity of the pure 6-sulfo, 6'-sulfo, or 6,6'-bissulfo sialyl Lewis X determinant with hitherto known anti-sialyl Lewis X antibodies strongly suggested 6-sulfo sialyl Lewis X to be the best candidate for the major sulfated sialyl Lewis X determinant on HEV, followed by 6,6'-bissulfo sialyl Lewis X, whereas 6'-sulfo sialyl Lewis X was unlikely. We newly generated monoclonal antibodies (mAbs) G152 and G72 directed against 6-sulfo sialyl Lewis X, which intensely labeled HEV in immunohistochemical examination and inhibited binding of recombinant L-selectin-IgG to HEV, suggesting that the determinant serves as a ligand for L-selectin. To test the concomitant expression of 6, 6'-bissulfo sialyl Lewis X, specific mAbs (G2706, G27011, G27037, and G27039) were generated, but all antibodies failed to react to HEV. Next, we established mAbs (AG97 and AG273) directed against 6-sulfo Lewis X, the asialo form of 6-sulfo sialyl Lewis X. The antibodies were not reactive to untreated HEV, but strongly reacted to si...Continue Reading

References

Jan 15, 1991·Carbohydrate Research·A KameyamaA Hasegawa
Sep 16, 1991·Biochemical and Biophysical Research Communications·A TakadaR Kannagi
May 19, 1995·The Journal of Biological Chemistry·S HemmerichS D Rosen
Apr 26, 1994·Biochemistry·S Hemmerich, S D Rosen

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Citations

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Mar 6, 2002·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Inbam IndrasinghSelvakumar Vettivel
Dec 5, 2002·Current Opinion in Structural Biology·Reiji Kannagi
Nov 13, 2003·Bioorganic & Medicinal Chemistry Letters·Kenji SasakiKazukiyo Kobayashi
Sep 29, 1999·Trends in Biochemical Sciences·T Feizi, C Galustian
Dec 22, 1999·Current Opinion in Chemical Biology·M B Lawrence
Jul 2, 2004·Organic Letters·Matthew R Pratt, Carolyn R Bertozzi
Sep 29, 2012·Nature Reviews. Immunology·Jean-Philippe GirardReinhold Förster
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Sep 17, 2009·Molecular BioSystems·Richard D Cummings
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Nov 5, 2003·The Journal of Experimental Medicine·Annemieke van ZanteSteven D Rosen
Sep 16, 2000·Glycobiology·S Hemmerich, S D Rosen
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Mar 23, 2004·Annual Review of Immunology·Steven D Rosen

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