Identification of a major histocompatibility complex class I-restricted T-cell epitope in the tumour-associated antigen, 5T4.

Immunology
Irina RedchenkoMiles W Carroll

Abstract

5T4 is a surface glycoprotein expressed on placental trophoblasts and also on a wide range of human carcinomas. Its highly restricted expression on normal tissues and broad distribution on many carcinomas make 5T4 a promising target for cancer immunotherapy. In the current study, we set out to investigate whether a 5T4-specific cytotoxic T lymphocyte (CTL) repertoire exists in healthy individuals. CD4-depleted peripheral blood mononuclear cells (PBMCs) from blood donors were screened using an ex vivo interferon-gamma (IFN-gamma) enzyme-linked immunospot (ELISPOT) assay. A panel of overlapping peptides, spanning the full length of the 5T4 protein, was used as a source of antigen. In the process of screening, one out of 30 blood donors demonstrated a positive ex vivo IFN-gamma ELISPOT response to a single 5T4 peptide. A polyclonal T-cell line was derived from this donor by culturing PBMCs with autologous peptide-pulsed dendritic cells (DCs). The resulting polyclonal T-cell line and clones were tested in a 51Cr-release assay and by ELISPOT and were shown to be peptide specific. Furthermore, antigen-presenting cells (APCs), infected with a viral vector expressing 5T4, were able to stimulate IFN-gamma production by the peptide-speci...Continue Reading

References

Nov 1, 1992·British Journal of Cancer·T StarzynskaP L Stern
Jan 1, 1990·British Journal of Cancer·P J SouthallP L Stern
Jan 1, 1990·British Journal of Cancer·H JonesP Stern
Sep 27, 1996·International Journal of Cancer. Journal International Du Cancer·C J CarsbergP L Stern
Nov 22, 1997·Current Opinion in Immunology·B J Van den Eynde, P van der Bruggen
Dec 17, 1998·European Journal of Gastroenterology & Hepatology·T StarzynskaP L Stern
Nov 24, 1999·Immunological Reviews·R F Wang, S A Rosenberg
Jul 1, 1995·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·E. WrigleyP.L. Stern

❮ Previous
Next ❯

Citations

Jun 24, 2008·International Immunology·William H ShinglerRichard Harrop
Dec 14, 2011·Immunotherapy·Rui-Tao ZhangHoward L Kaufman
Jun 1, 2007·Biomarkers in Medicine·Adam E SnookScott A Waldman
Sep 5, 2008·Expert Review of Vaccines·Robert J Amato
Jan 22, 2010·Expert Opinion on Biological Therapy·Robert J Amato
Nov 8, 2008·Expert Opinion on Biological Therapy·Scott S Tykodi, John A Thompson
Aug 31, 2007·Expert Opinion on Biological Therapy·Robert J Amato
Oct 21, 2016·Cancer Immunology, Immunotherapy : CII·Peter L Stern, Richard Harrop
Sep 15, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Moreno Di MarcoStefan Stevanović
Jun 27, 2009·Immunopharmacology and Immunotoxicology·Junli WuYi Miao
Jun 19, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rachid BouchermaFrançois A Lemonnier
Oct 15, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael RasmussenSøren Buus
Mar 6, 2021·Human Vaccines & Immunotherapeutics·Yuexin XuScott S Tykodi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.