Identification of a major Listeria monocytogenes outbreak clone linked to soft cheese in Northern Italy - 2009-2011

BMC Infectious Diseases
Ettore AmatoMirella M Pontello

Abstract

Molecular subtyping and enhanced surveillance in Lombardy region identified a cluster of possibly related listeriosis cases from 2006 to 2010. This cluster grouped 31 isolates that belonged to serotype 1/2a and Sequence Type 38 (ST38) as defined by Multilocus Sequence Typing (MLST). Our study expanded the previous investigation to include cases from 2011 to 2014 and used Multi-Virulence-Locus Sequence Typing (MVLST) on all ST38 isolates to better understand their epidemiology and possibly identify a common source outbreak. Out of 306 L. monocytogenes clinical isolates collected, 43 (14.1%) belonged to ST38 with cases occurring in nine out of twelve Lombardy provinces. The ST38 isolates were split by MVLST into two Virulence Types (VTs): VT80 (n = 12) and VT104 (n = 31). VT104 cases were concentrated between 2009 and 2011 in two provinces, Bergamo and Milan. An epidemiologic investigation was performed and in one case, a matching VT104 isolate was retrieved from a soft cheese sample from a patient's refrigerator. Our findings revealed a major listeriosis outbreak in Northern Italy linked to soft cheese in 2009-2011, which went undetected by local health authorities. Our study shows that integrating subtyping methods with convent...Continue Reading

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Citations

Sep 22, 2018·FEMS Microbiology Letters·F G MelgaçoM P Miagostovich
May 30, 2019·Frontiers in Microbiology·Xiaoai ZhangXiaochen Ma
Oct 24, 2020·Critical Reviews in Food Science and Nutrition·Akbar BahramiLeonard Williams

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Methods Mentioned

BETA
Electrophoresis

Software Mentioned

MVLST
MEGA
Molecular Evolutionary Genetic Analysis software ( MEGA )
MLST

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