Identification of a membrane protein associated with expression of the surface exclusion region of the F transfer operon.

Journal of Bacteriology
E G Minkley, K Ippen-Ihler

Abstract

Membrane preparations from radioactively labeled male and female strains of Escherichia coli K-12 were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. An intensely labeled band corresponding to a protein of molecular weight of 24,000 was readily apparent in preparations from Hfr and F-prime strains but not in those from female strains. When preparations from a series of Hfr strains containing transfer operon deletions were examined, presence of the band was found to be associated with retention of the region of the F transfer operon between ilzA and traD. Thus, the band ("protein S") appears to be the product of an F tra operon activity corresponding to traS (the gene for surface or entry exclusion), or an unknown gene in its vicinity. As predicted, protein S was subject to Fin+ control; only a faint band was detectable if the repressed plasmid R100 was also present in the F lac strain. A 24,000-dalton protein was also found in membrane preparations from strains carrying the derepressed plasmids R100-1 and R1-19 but not in those from strains carrying the repressed plasmids R100 or R1. Thus, the appearance of protein S in the membrane may be a general phenomenon resulting from transfer ...Continue Reading

References

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Citations

Jan 1, 1982·Molecular & General Genetics : MGG·D MooreK Ippen-Ihler
Apr 1, 1986·European Journal of Biochemistry·S NyströmA Wieslander
Jan 1, 1984·Molecular & General Genetics : MGG·E G Minkley, N S Willetts
Apr 1, 1981·Journal of Bacteriology·D MooreK Ippen-Ihler
Jun 1, 1994·Microbiological Reviews·L S FrostR A Skurray
Aug 1, 1986·Journal of Bacteriology·E A WorobecW Paranchych
Apr 29, 2008·Plasmid·M Pilar Garcillán-Barcia, Fernando de la Cruz

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