PMID: 6540600Aug 8, 1984Paper

Identification of a Na+,K+, Cl--cotransport protein of Mr 34 000 from kidney by photolabeling with [3H]bumethanide. The protein is associated with cytoskeleton components

Biochimica Et Biophysica Acta
P L JørgensenW D Rees

Abstract

A polypeptide of Mr 34 000 is photolabeled with [3H]bumethanide after binding of this drug to membranes from the outer renal medulla and irradiation at 345 nm, a wavelength where bumethanide has an absorption maximum. Our data show that the polypeptide of Mr 34 000 is a component of the Na+/K+/Cl--cotransport system. The [3H]bumethanide binding protein is not extracted by concentrations of the nonionic detergent C12E8 that solubilizes 67% of the protein of the membranes including (Na+ + K+)-ATPase. This step increases the capacity for binding of [3H]bumethanide to 681 pmol/mg protein. Extraction of the binding protein requires high ionic strength suggesting that the Na+/K+/Cl--cotransport protein is associated with cytoskeleton components. This association may be important for control of the entry of NaCl into the cytoplasm and for cellular regulation of the rate of active transport of NaCl across the tubule cells in the thick ascending limb of Henles loop.

References

Mar 1, 1980·The American Journal of Physiology·H C PalfreyP Greengard
Jul 1, 1980·Physiological Reviews·P L Jørgensen
Aug 11, 1983·Biochimica Et Biophysica Acta·B Geiger

❮ Previous
Next ❯

Citations

Nov 1, 1991·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·M L MelziA Aperia
Nov 1, 1989·The Journal of Membrane Biology·J N George, R J Turner
Apr 1, 1988·The Journal of Membrane Biology·R J Turner, J N George
Jan 1, 1986·Acta Physiologica Scandinavica·O Eriksson, P J Wistrand
Jun 1, 1991·The Journal of Clinical Investigation·M ShapiroJ L Madara
Oct 1, 1995·Kidney International·H F Cantiello
Jan 1, 1985·Annals of the New York Academy of Sciences·R KinneB König
Jan 1, 1986·Archives of Oral Biology·M KawaguchiB J Baum
Oct 19, 1999·Microscopy Research and Technique·J H Henson
Jul 26, 1996·The Journal of Biological Chemistry·B K BerdievI I Ismailov
Dec 11, 1997·The Journal of Experimental Zoology·H F Cantiello
Nov 1, 1990·Journal of Cellular Physiology·J J Kort, G Koch
Jan 1, 1986·The Journal of Membrane Biology·P Geck, E Heinz
Nov 30, 2005·Journal of Pineal Research·Gloria Benítez-King
Jan 5, 2000·Physiological Reviews·J M Russell
Aug 1, 1994·The American Journal of Physiology·W SuvitayavatM C Rao
May 1, 1986·The American Journal of Physiology·K Amsler, R Kinne
May 1, 1986·The American Journal of Physiology·E K HoffmannP Dunham
Apr 1, 1986·The American Journal of Physiology·P B Dunham, P J Logue
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·D A Klaerke, P L Jørgensen
Jan 31, 1991·Biochimica Et Biophysica Acta·K G EngströmJ Sehlin
Mar 13, 2003·Kidney International·Gerardo Ramírez-RodríguezGloria Benítez-King
Jan 1, 1986·Kidney International·P L Jørgensen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.