PMID: 8592153Feb 1, 1996Paper

Identification of a neuropeptide and neuropeptide-processing enzymes in aqueous humor confers neuroendocrine features to the human ocular ciliary epithelium

Journal of Neurochemistry
J OrtegoM Coca-Prados

Abstract

The ocular ciliary epithelium, the site of aqueous humor secretion in the mammalian eye, is believed to play a key function in signaling mechanisms that regulate the rate of secretion, and thus intraocular pressure. One possible way of mediating these signaling functions is through neuropeptides and hormones secreted into the aqueous humor and acting on target tissues. We recently identified a cDNA clone sharing 100% identity with carboxypeptidase E (CPE), a neuropeptide-processing enzyme. Utilizing polymerase chain reaction, we further identified and characterized another processing enzyme, the peptidylglycine alpha-amidating monooxygenase (PAM), and the neuropeptide secretogranin II, a molecular marker restricted to neuroendocrine tissues. Using specific probes, we found that the nonpigmented ciliary epithelial cells express CPE, PAM, and secretogranin II mRNA, and protein. We also found that CPE and secretogranin II are abundant in aqueous humor. Treatment of cultured ciliary epithelial cells with veratridine and phorbol ester up-regulates CPE and PAM. Secretogranin II was found to be induced by veratridine, whereas phorbol ester had little effect, suggesting different mechanisms for secretion. The results demonstrate that s...Continue Reading

Citations

Sep 21, 2007·Current Eye Research·Rubens Bertazolli-FilhoEduardo Miguel Laicine
Jan 15, 2010·Journal of Ocular Biology, Diseases, and Informatics·Katja N VolpertPaul G Layer
Apr 5, 2005·Current Eye Research·Shay-Whey KohEve Higginbotham
May 1, 1999·Biochemical and Biophysical Research Communications·J Ortego, M Coca-Prados
Jun 8, 2001·Biochemical and Biophysical Research Communications·R Bertazolli-FilhoM Coca-Prados
Apr 7, 1999·Progress in Retinal and Eye Research·M Coca-PradosJ Ortego
Jun 13, 2006·Molecular and Cellular Endocrinology·Sikha GhoshMiguel Coca-Prados

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