Identification of a new anti-LPS agent, geniposide, from Gardenia jasminoides Ellis, and its ability of direct binding and neutralization of lipopolysaccharide in vitro and in vivo

International Immunopharmacology
Xinchuan ZhengJiang Zheng

Abstract

Lipopolysaccharide (LPS/endotoxin) is a key pathogen recognition molecule for sepsis. Currently, one of the therapeutic approaches for severe sepsis is focusing on the neutralization of LPS, and clinical trials have shown a lot of traditional Chinese herbs possess anti-sepsis function. Herein, to elucidate the bioactive components of traditional Chinese herbs that can neutralize LPS, the lipid A-binding abilities of sixty herbs were tested using affinity biosensor technology. The aqueous extract of Gardenia jasminoides Ellis, traditionally used to treat inflammation in Asian countries for centuries, was further investigated. Subsequently, a monomer, identified as geniposide, was isolated. In vitro, geniposide was found to directly bind LPS and neutralize LPS. It dose-dependently inhibited cytokines release from RAW264.7 cells induced by LPS without affecting the cell viability, and inhibited TNF-α mRNA expression up-regulated by LPS. However, geniposide did not decrease TNF-α release induced by CpG DNA, Poly I:C or IL-1β. Significantly, geniposide dose-dependently down-regulated TLR4 mRNA expression up-regulated by LPS, and suppressed the phosphorylations of p38 MAKP induced by LPS but not by IL-1β. In vivo, geniposide (40mg/kg...Continue Reading

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Citations

Oct 3, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Danae Carrillo-OcampoVerónica Rodríguez-López
Jan 2, 2016·Journal of Ethnopharmacology·Hsing-Yu ChenYu-Chun Chen
May 15, 2012·Ultrasonics Sonochemistry·Xin-Sheng WangYing Shao
Jul 1, 2014·British Journal of Pharmacology·Prashant ShuklaPrabhat Ranjan Mishra
Sep 27, 2015·European Journal of Pharmacology·YiMei ChenChristian Hölscher
Dec 2, 2014·International Immunopharmacology·Jin-Yun ChenJian Chen
May 24, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Yan-Xi ZhouCheng Peng
Nov 6, 2018·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jullyana S S QuintansLucindo J Quintans-Júnior

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