PMID: 9542912May 23, 1998Paper

Identification of a new DNA region specific for members of Mycobacterium tuberculosis complex

Journal of Clinical Microbiology
J MagdalenaCamille Locht

Abstract

The successful use of DNA amplification for the detection of tuberculous mycobacteria crucially depends on the choice of the target sequence, which ideally should be present in all tuberculous mycobacteria and absent from all other bacteria. In the present study we developed a PCR procedure based on the intergenic region (IR) separating two genes encoding a recently identified mycobacterial two-component system named SenX3-RegX3. The senX3-regX3 IR is composed of a novel type of repetitive sequence, called mycobacterial interspersed repetitive units (MIRUs). In a survey of 116 Mycobacterium tuberculosis strains characterized by different IS6110 restriction fragment length polymorphisms, 2 Mycobacterium africanum strains, 3 Mycobacterium bovis strains (including 2 BCG strains), and 1 Mycobacterium microti strain, a specific PCR fragment was amplified in all cases. This collection included M. tuberculosis strains that lack IS6110 or mtp40, two target sequences that have previously been used for the detection of M. tuberculosis. No PCR fragment was amplified when DNA from other organisms was used, giving a sensitivity of 100% and a specificity of 100% in the confidence limit of this study. The numbers of MIRUs were found to vary a...Continue Reading

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Citations

Jun 13, 2000·Journal of Bacteriology·T C Zahrt, V Deretic
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·E MazarsP Supply
Dec 16, 2011·Journal of Clinical Microbiology·Jessica L de BeerUNKNOWN Global Network for the Molecular Surveillance of Tuberculosis 2009
Oct 26, 2014·BMC Microbiology·Dalin RifatPetros C Karakousis
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·Daniel J BretlThomas C Zahrt
Jul 12, 2008·PLoS Neglected Tropical Diseases·Zoheira DjelouadjiMichel Drancourt
Feb 11, 2016·Jundishapur Journal of Microbiology·Mohammad Hasan AelamiJalal Mardaneh
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May 17, 2014·Research in Veterinary Science·E GormleyS Rodriguez-Campos
Sep 13, 2001·Trends in Microbiology·R BroschS T Cole
Jul 21, 2001·Tuberculosis·S V GordonR G Hewinson
Nov 8, 2005·Journal of Clinical Microbiology·Kristin KremerDick van Soolingen
Feb 10, 2004·Journal of Clinical Microbiology·Sayera BanuRoland Brosch
Jan 5, 2008·Journal of Clinical Microbiology·Maegan AshworthGerard A Cangelosi
Sep 22, 2006·Journal of Clinical Microbiology·Nicoletta LariCarlo Garzelli

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