PMID: 11335114May 4, 2001Paper

Identification of a new gene family specifically expressed in chicken embryonic stem cells and early embryo

Mechanisms of Development
Hervé AcloqueJacques Samarut

Abstract

Chicken embryonic stem (CES) cells are pluripotent cells derived from chicken early blastoderm. In order to identify new genes specifically expressed in these pluripotent cells, we have used a gene trap strategy and cloned a novel gene family called cENS for chicken Embryonic Normal Stem cell gene. The cENS genes expression decreases after induction of CES cells differentiation in culture and is restricted in vivo to the very early embryo. We have characterized three different cENS genes. One, cENS-1, is composed of an open reading frame inserted between two terminal direct repeats which are the common point of the cENS genes. cENS-1 encodes a protein identical to cERNI, a recently described protein. cENS-2 is a truncated form of cENS-1. cENS-3 presents two adjacent open reading frames coding respectively for env and pol related proteins. The presence of conserved direct repeats, of retrovirus related genes and the absence of introns argue in favor of a retroviral origin of the cENS genes. In the cENS we identified a promoter region whose activity is strong in CES cells and decreases after induced differentiation showing a highly specific transcriptional activity specific of undifferentiated chicken embryonic stem cells.

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Citations

Mar 31, 2005·Biochemical and Biophysical Research Communications·Masaharu SatoHaruo Matsuda
Aug 30, 2005·Nature Biotechnology·Lei ZhuRobert J Etches
Apr 27, 2004·Nucleic Acids Research·Hervé AcloqueJacques Samarut
Jul 17, 2004·Genome Research·Thomas WickerRobert Ivarie
Dec 31, 2009·Development, Growth & Differentiation·Fabrice Lavial, Bertrand Pain
Oct 18, 2012·MBio·Mohan BolisettyKaren Beemon
Jan 11, 2008·PLoS Biology·Costis PapanayotouClaudio D Stern
May 1, 2007·Regenerative Medicine·Lan Dang, Vincent Tropepe
Jan 26, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hervé AcloqueBertrand Pain
Dec 6, 2005·Mechanisms of Development·Marie-Cecile van de LavoirRobert J Etches
Oct 10, 2013·Stem Cell Research·Sittipon Intarapat, Claudio D Stern
Jan 3, 2013·Development, Growth & Differentiation·Christian JeanBertrand Pain
Mar 3, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Paul E Mozdziak, James N Petitte
Jan 24, 2008·Molecular Reproduction and Development·Philip A LeightonRobert J Etches
Jun 5, 2018·Stem Cells International·Li ZhangHongbing Han
Apr 15, 2005·Development·Claudio D Stern

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