Identification of a new HLA-A*0201-restricted cytotoxic T lymphocyte epitope from CML28

Cancer Immunology, Immunotherapy : CII
Jun-Feng HanYu-Zhang Wu

Abstract

Identification of cytotoxic T lymphocyte (CTL) epitopes from additional tumor antigens is essential for the development of specific immunotherapy of malignant tumors. CML28, a recently discovered cancer-testis (CT) antigen from chronic myelogenous leukemia, is considered to be a promising target of tumor-specific immunotherapy. Because HLA-A*0201 is one of the most common histocompatibility molecule in Chinese, we aim at identifying CML28 peptides presented by HLA-A*0201. A panel of CML28-derived antigenic peptides was predicted using a computer-based program. Four peptides with highest predicted score were synthesized and tested for their binding affinities to HLA-A*0201 molecule. Then these peptides were assessed for their immunogenicity to elicit specific immune responses mediated by CTLs both in vitro, from PBMCs sourced from four healthy HLA-A*0201(+) donors, and in vivo, in HLA-A*0201 transgenic mice. One of the tested peptides, CML28((173-181)), induced peptide-specific CTLs in vitro as well as in vivo, which could specifically secrete IFN-gamma and lyse major histocompatibility complex (MHC)-matched tumor cell lines endogenously expressing CML28 antigen and CML28((173-181) )pulsed Jurkat-A2/Kb cells, respectively. These...Continue Reading

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Citations

Jun 12, 2013·Journal of Neuro-oncology·Zhao YangQingwu Yang
Apr 4, 2012·Expert Opinion on Drug Discovery·Violeta I Pérez-Nueno, David W Ritchie
Feb 21, 2008·European Journal of Haematology·Jochen Greiner, Michael Schmitt
Apr 2, 2009·Cancer Control : Journal of the Moffitt Cancer Center·Javier Pinilla-IbarzJason A Dubovsky
Jan 11, 2013·Cancer Control : Journal of the Moffitt Cancer Center·Jason B Brayer, Javier Pinilla-Ibarz

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