Identification of a new intermediate state that binds but not activates transducin in the bleaching process of bovine rhodopsin

FEBS Letters
S TachibanakiYoshinori Shichida

Abstract

Using time-resolved low-temperature spectroscopy, we have examined whether or not bovine rhodopsin has a unique transducin-binding state, meta Ib, previously detected from chicken rhodopsin. Unlike chicken meta Ib, bovine meta Ib was detected only by detailed kinetics analysis of the bleaching process, but it was stabilized by transducin and visualized in the observed spectral changes. From the effect of GTPgammaS, it was revealed that meta Ib induced no GDP-GTP exchange reaction in transducin. Thus meta Ib is a common intermediate of vertebrate rhodopsin and transducin is activated in two steps by meta Ib and meta II.

References

Dec 1, 1992·Photochemistry and Photobiology·T OkanoT Yoshizawa
Oct 5, 1990·Science·R R FrankeK P Hofmann
Mar 30, 1963·Nature·T YOSHIZAWA, G WALD
Nov 1, 1963·The Journal of General Physiology·R G MATTHEWSG WALD

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Citations

May 9, 2012·The Journal of Physical Chemistry. B·Markus EilersSteven O Smith
Nov 13, 2002·The Journal of Biological Chemistry·Martin HeckKlaus Peter Hofmann
Jul 23, 2008·Photochemistry and Photobiology·Takefumi MorizumiYoshinori Shichida
Mar 10, 2009·Photochemistry and Photobiology·Mònica AguilàPere Garriga
Dec 25, 2013·Chemical Reviews·Oliver P ErnstHideki Kandori
Jun 28, 2006·Photochemistry and Photobiology·Yoshinori Shichida, Takefumi Morizumi
Apr 29, 2010·The Biochemical Journal·Beata JastrzebskaKrzysztof Palczewski
Mar 5, 2004·Chembiochem : a European Journal of Chemical Biology·Masaji IshiguroTakahiro Hirano
Jan 14, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Yoshinori Shichida, Takahiro Yamashita
Sep 16, 2004·Chembiochem : a European Journal of Chemical Biology·Masaji Ishiguro
Jan 1, 2014·The Journal of Biological Chemistry·Keiichi KojimaYoshinori Shichida

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