Identification of a new motif for CDPK phosphorylation in vitro that suggests ACC synthase may be a CDPK substrate

Archives of Biochemistry and Biophysics
Cinta Hernández SebastiàS C Huber

Abstract

1-Amino-cyclopropane-1-carboxylate synthase (ACS) catalyzes the rate-determining step in the biosynthesis of the plant hormone ethylene, and there is evidence for regulation of stability of the protein by reversible protein phosphorylation. The site of phosphorylation of the tomato enzyme, LeACS2, was recently reported to be Ser460, but the requisite protein kinase has not been identified. In the present study, a synthetic peptide based on the known regulatory phosphorylation site (KKNNLRLS460FSKRMY) in LeACS2 was found to be readily phosphorylated in vitro by several calcium-dependent protein kinases (CDPKs), but not a plant SNF1-related protein kinase or the kinase domain of the receptor-like kinase, BRI1, involved in brassinosteroid signaling. Studies with variants of the LeACS2-Ser460 peptide establish a fundamentally new phosphorylation motif that is broadly targeted by CDPKs: phi -1-[ST]0- phi +1-X-Basic+3-Basic+4, where phi is a hydrophobic residue. Database analysis using the new motif predicts a number of novel phosphorylation sites in plant proteins. Finally, we also demonstrate that CDPKs and SnRK1s do not recognize motifs presented in the reverse order, indicating that side chain interactions alone are not sufficien...Continue Reading

References

Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D E WrightV Chau
Dec 1, 1994·Plant Molecular Biology·T I Zarembinski, A Theologis
Dec 1, 1993·Archives of Biochemistry and Biophysics·R W McMichaelS C Huber
May 12, 2000·Plant Physiology·D ToroserS C Huber
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·I HwangJ F Harper
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A B Bleecker, H Kende
Jun 14, 2003·Plant Physiology·Estelle M HrabakAlice C Harmon
Aug 28, 2003·The Plant Journal : for Cell and Molecular Biology·Shane C HardinSteven C Huber
Sep 12, 2003·The Journal of Biological Chemistry·Takeshi YamagamiAthanasios Theologis
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Steven D. Clouse, Jenneth M. Sasse

❮ Previous
Next ❯

Citations

Jun 14, 2005·Trends in Plant Science·Hyun Sook Chae, Joseph J Kieber
Aug 3, 2005·Nature Reviews. Molecular Cell Biology·Jeffrey F Harper, Alice Harmon
Jun 7, 2008·Journal of Experimental Botany·I El-SharkawyD C W Brown
Jul 2, 2009·Journal of Experimental Botany·Zhefeng LinDon Grierson
Aug 15, 2013·Journal of Experimental Botany·Shih-Jhe HuangIng-Feng Chang
Apr 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ryoung ShinDaniel P Schachtman
Jul 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Andrea A LudwigTina Romeis
Sep 15, 2012·Trends in Plant Science·Marie Boudsocq, Jen Sheen
Sep 24, 2008·The Plant Journal : for Cell and Molecular Biology·Matthew J ChristiansRichard D Vierstra
Nov 5, 2008·The Plant Journal : for Cell and Molecular Biology·Maureen HansenJoseph J Kieber
Mar 28, 2008·The Plant Journal : for Cell and Molecular Biology·Florina VladSylvain Merlot
Jul 28, 2010·The Plant Journal : for Cell and Molecular Biology·Yusuke KamiyoshiharaHitoshi Mori
May 26, 2010·The Plant Journal : for Cell and Molecular Biology·Norbert MehlmerMarkus Teige
Apr 18, 2014·The Plant Journal : for Cell and Molecular Biology·Xingju LuoZhizhong Gong
Oct 21, 2015·Frontiers in Plant Science·Li Song, Dong Liu
Dec 17, 2009·The Biochemical Journal·Thomas A DeFalcoWayne A Snedden
Jun 17, 2009·The Biochemical Journal·Shane C HardinSteven C Huber
Dec 3, 2014·Plant Science : an International Journal of Experimental Plant Biology·Hui MaYan Xiang
May 10, 2012·Critical Reviews in Biotechnology·Maryam SarwatXiangyang Hu
Jun 23, 2015·Molecules and Cells·Gyeong Mee Yoon
Jul 9, 2014·Journal of Zhejiang University. Science. B·Chang-ying LiuMao-de Yu
Apr 10, 2015·Journal of Plant Physiology·Ajay Kumar Dixit, Chelliah Jayabaskaran
Dec 19, 2014·Frontiers in Plant Science·Maria A RodriguesLuciano Freschi
Apr 26, 2017·The Plant Journal : for Cell and Molecular Biology·Han Yong LeeGyeong Mee Yoon
Nov 22, 2017·International Journal of Molecular Sciences·Wuwu Xu, Wenchao Huang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.