Identification of a new pathogen-induced member of the subtilisin-like processing protease family from plants.

The Journal of Biological Chemistry
P TorneroP Vera

Abstract

By using biochemical, immunological, and molecular strategies we have identified and cloned a cDNA encoding a protease from tomato (Lycopersicon esculentum) plants (P69B) that is part of a proteolytic system activated in the plant as a result of infection with citrus exocortis viroid. This new protease is closely related, in terms of amino acid sequence and structural organization, to the previously identified pathogenesis-related subtilisin-like protease (Tornero, P., Conejero, V., and Vera, P. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 6332-6337). The 745-residue amino acid sequence of P69B begins with a cleavable signal peptide, contains a prodomain and a 631-residue mature domain which is homologous to the catalytic modules of bacterial subtilisins and eukaryotic Kex2-like proteases. Within the catalytic domain, the essential Asp, His, and Ser residues that conform the catalytic triad of this family of proteases are conserved in P69B. Northern blot and reverse transcriptase-polymerase chain reaction analysis demonstrated widespread induced expression of the 2.5-kilobase hybridizing mRNA in plant tissues as a consequence of viroid infection. We propose that P69B is a member of a complex gene family of plant Kex2/subtilisin-l...Continue Reading

References

Oct 1, 1992·Current Opinion in Cell Biology·W T Chen
Jan 1, 1990·Annual Review of Biochemistry·D J Bowles
May 15, 1987·Analytical Biochemistry·J LogemannL Willmitzer
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R S FullerJ Thorner
Oct 1, 1989·European Journal of Biochemistry·I RodrigoV Conejero
Feb 1, 1986·Analytical Biochemistry·M Hattori, Y Sakaki
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Oct 1, 1994·Trends in Biochemical Sciences·B Kobe, J Deisenhofer
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·A Schaller, C A Ryan
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·V PautotL L Walling
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·N G SeidahR Day
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·P TorneroP Vera
Aug 1, 1996·The Plant Journal : for Cell and Molecular Biology·P TorneroP Vera

❮ Previous
Next ❯

Citations

Oct 1, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Narayanan N NarayananMadan K Bhattacharyya
Jan 17, 2012·Functional & Integrative Genomics·Andreia FigueiredoMaria Salomé Pais
Jul 31, 2004·Plant Physiology and Biochemistry : PPB·Neel G BarnabyAnna Tan-Wilson
Jul 12, 2005·Plant Physiology and Biochemistry : PPB·Cecília M Antão, F Xavier Malcata
Oct 22, 2002·FEBS Letters·Tatjana PopovicJoze Brzin
Apr 4, 2000·Phytochemistry·K ArimaM Kaneda
Aug 17, 2001·Biochimie·A M BogachevaB N Golovkin
Jan 29, 2000·The Plant Journal : for Cell and Molecular Biology·E MaydaP Vera
Apr 9, 2008·Molecular Plant-microbe Interactions : MPMI·Laurent BrechenmacherGary Stacey
May 3, 2008·The Plant Cell·Mohammed ShababRenier A L van der Hoorn
Sep 19, 2006·Biochemistry. Biokhimii︠a︡·V V Mosolov, T A Valueva
Feb 22, 2014·Nature Communications·Chen ChenHong-Xuan Lin
Jul 18, 2002·Physiologia Plantarum·Vadim BeilinsonNiels C Nielsen
May 15, 2012·Biochimica Et Biophysica Acta·Jaruwan SiritapetaweeSompong Thammasirirak
Aug 19, 2007·The Plant Journal : for Cell and Molecular Biology·Eleanor M GilroyPaul R J Birch
Jul 15, 2006·Phytochemistry·Subhash C YadavM V Jagannadham
Sep 6, 2011·Biotechnology Advances·Nuria González-RábadeMaría Del Carmen Oliver-Salvador
Apr 23, 2015·Plant Physiology and Biochemistry : PPB·Jaruwan SiritapetaweeSompong Klaynongsruang
Feb 23, 2010·Plant Signaling & Behavior·Rolf RoseChristian Ottmann
Dec 9, 2014·Plant Physiology and Biochemistry : PPB·María Belén FernándezMaría Gabriela Guevara
Jan 8, 2015·Frontiers in Plant Science·Andreia FigueiredoMónica Sebastiana
Feb 22, 2017·Genetics and Molecular Biology·Thais P SouzaMarcio C Silva-Filho

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.