PMID: 2497051Feb 1, 1989Paper

Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis

Genes & Development
D X SunP Setlow

Abstract

During sporulation of Bacillus subtilis, two identical genomes segregate in two compartments, the forespore and mother cell. These genomes are expressed differentially, with some genes such as sspE turned on only in the forespore. In vitro transcription of sspE was obtained only with RNA polymerase extracted from sporulating cells. Fractionation of factors associated with this enzyme and reconstitution with core RNA polymerase from vegetative cells generated an enzyme accurately transcribing sspE in vitro and led to purification of a polypeptide with the amino-terminal sequence of the spoIIIG product. Inactivation of spoIIIG abolished expression of sspE and five other forespore-specific genes, whereas synthesis of the spoIIIG product in vegetative cells rapidly turned these genes on. Therefore, spoIIIG encodes a sigma-factor, sigma G, which controls the expression of multiple genes in the forespore compartment.

References

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Citations

Feb 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J W Gober
May 25, 1991·Gene·J J WuC P Moran
Mar 24, 2004·Nature Reviews. Microbiology·Jeff Errington
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R SchmidtR Losick
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S LuL Kroos
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A Driks, R Losick
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y F Chen, J D Helmann
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L Duncan, R Losick
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·P J LewisJ Errington
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·M L KarowP J Piggot
Sep 25, 2007·Journal of Bacteriology·William H ColemanPeter Setlow
Sep 11, 2012·Journal of Bacteriology·Fernando H Ramírez-GuadianaMario Pedraza-Reyes
Aug 4, 2005·Journal of Bacteriology·Venkata Ramana Vepachedu, Peter Setlow
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Justin S LenhartLyle A Simmons
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·W L NicholsonP Setlow
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·David W Hilbert, Patrick J Piggot
Jul 13, 2006·Annual Review of Biomedical Engineering·Kenneth R Diller
Jan 1, 1996·Annual Review of Genetics·P Stragier, R Losick
Mar 28, 2009·International Journal of Molecular Sciences·Norma Ramírez-RamírezMario Pedraza-Reyes
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·H KovacsM D Yudkin
Mar 1, 1991·Molecular Microbiology·S R PartridgeJ Errington
Nov 1, 1990·Molecular Microbiology·P Stragier, R Losick
Mar 1, 1992·Molecular Microbiology·J Errington, N Illing

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