Identification of a new strain of mouse kidney parvovirus associated with inclusion body nephropathy in immunocompromised laboratory mice.

Emerging Microbes & Infections
Zhongming GeJames G Fox

Abstract

Inclusion body nephropathy (IBN) and kidney fibrosis in aged immunodeficient mice and, to lesser extent, in immunocompetent mice have been recently linked to infection of mouse kidney parvovirus (MKPV), also known as murine chapparvovirus (MuCPV). Knowledge about its prevalence and the complete genome sequence of more MKPV strains is essential for understanding phylogenetic relationships and pathogenicity among MKPV strains. In the present study using PCR and genome walking, we determined the complete 4440-nucleotide genome of a new MKPV strain, namely MIT-WI1, which was identified in IBN-affected Il2rg-/-Rag2-/- c-Kit W-sh/W-sh mice housed in the vivarium at Whitehead Institute for Biomedical Research (WI). The overall nucleotide (>94%) and deduced amino acid sequences (>98%) of p10, p15, NS1 (replicase), NS2 and VP1 (capsid protein) within the MIT-WI1 genome, are closely related to MKPV/MuCPV strains described in laboratory and wild Mus musculus mice. In addition, PCR and qPCR assays using newly designed primers conserved among the known MKPV/MuCPV genomes were developed and utilized to assess MKPV status in selected laboratory mice. MKPV was also detected in immunodeficient (NSG) and immunocompetent (Crl:CD1(ICR), UTXflox) m...Continue Reading

References

Apr 18, 2012·Nature Reviews. Nephrology·Dirk R J Kuypers
Jan 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Malkiel A CohenRudolf Jaenisch
Nov 3, 2014·Annual Review of Virology·Susan F Cotmore, Peter Tattersall
Oct 25, 2016·Transplantation·Caroline LamarcheJean-Sébastien Delisle
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Apr 19, 2018·MBio·Simon H WilliamsW Ian Lipkin
Aug 31, 2019·GeroScience·Jessica M SnyderWarren Ladiges

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Citations

Oct 30, 2020·Journal of Virology·Yanpeng LiEric Delwart
Feb 10, 2021·Transboundary and Emerging Diseases·Federica Di ProfioBarbara Di Martino

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Datasets Mentioned

BETA
MT093738
MG679365.1

Methods Mentioned

BETA
PCR

Software Mentioned

RNAscope
Blastn
DNAStar Lasergene 13
DNASTAR Lasergene
DNAStar Lasergene package
GraphPad Prism

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