Identification of a novel alternatively spliced mRNA of murine pulmonary surfactant protein B

American Journal of Respiratory Cell and Molecular Biology
X ChiH R Colten

Abstract

An alternatively spliced mRNA of pulmonary surfactant protein B (SP-B) was identified in murine lung. Sequencing analysis revealed a 69 base-pair (bp) deletion at the beginning of exon 7 of SP-B, presumably the result of an alternative splicing event. Reverse transcription-polymerase chain reaction (RT-PCR) of mouse, rat, and rabbit lung RNA revealed the existence of full-length and the 69-bp deleted short form. Ribonuclease protection assay of the SP-B messenger RNA (mRNA) demonstrated expression of both isoforms in five strains of adult and fetal mice with different genetic backgrounds, as well as in rabbit, but not in human. Splice junction sequences in exon 6 and at the exon 7 splice boundary for the two isoforms are similar, including AG doublet identity, but sequence differences do not account for species variation in isoform abundance. The abundance of the short SP-B mRNA isoform was approximately 30% of total SP-B mRNA in mouse and rabbit. Analysis of precursor SP-B protein in mouse lung suggested that the two mRNA species are expressed as stable protein isoforms.

References

Jan 1, 1989·Annual Review of Genetics·C W SmithB Nadal-Ginard
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S W GlasserJ A Whitsett
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S HawgoodR T White
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·J C ClarkJ A Whitsett
Aug 1, 1994·American Journal of Respiratory Cell and Molecular Biology·D E deMelloH R Colten
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K A WikenheiserJ A Whitsett
Feb 11, 1993·The New England Journal of Medicine·L M NogeeH R Colten
Mar 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L BalvayM Y Fiszman

❮ Previous
Next ❯

Citations

Jul 23, 2015·Journal of Cell Communication and Signaling·Elger MartenChristiane E L Dammann
Jan 24, 2017·Nature Methods·Xiaojie QiuCole Trapnell
Oct 20, 1999·The Annals of Otology, Rhinology, and Laryngology·J M DuttonJ M Snyder
Feb 1, 2000·The Journal of Biological Chemistry·D C BeckT E Weaver
Apr 6, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·S M Pietschmann, U Pison
Nov 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·L I Gobran, S A Rooney
Oct 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·J A GutierrezJ R Fineman
Feb 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Marlene StrayerPhilip L Ballard
Oct 21, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Laurice I Gobran, Seamus A Rooney
Jun 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robert J HomerJack A Elias

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.