Identification of a novel cytochrome P450 CYP321B1 gene from tobacco cutworm (Spodoptera litura) and RNA interference to evaluate its role in commonly used insecticides

Insect Science
Rui-Long WangRen-Sen Zeng

Abstract

Insect cytochrome P450 monooxygenases (CYPs or P450s) play an important role in detoxifying insecticides leading to resistance in insect populations. A polyphagous pest, Spodoptera litura, has developed resistance to a wide range of insecticides. In the present study, a novel P450 gene, CYP321B1, was cloned from S. litura. The function of CYP321B1 was assessed using RNA interference (RNAi) and monitoring resistance levels for three commonly used insecticides, including chlorpyrifos, β-cypermethrin and methomyl. The full-length complementary DNA sequence of CYP321B1 is 1814 bp long with an open reading frame of 1 488 bp encoding 495 amino acid residues. Quantitative reverse-transcriptase polymerase chain reaction analyses during larval and pupal development indicated that CYP321B1 expression was highest in the midgut of fifth-instar larvae, followed by fat body and cuticle. The expression of CYP321B1 in the midgut was up-regulated by chlorpyrifos, β-cypermethrin and methomyl with both lethal concentration at 15% (LC15 ) (50, 100 and 150 μg/mL, respectively) and 50%(LC50 ) dosages (100, 200 and 300 μg/mL, respectively). Addition of piperonyl butoxide (PBO) significantly increased the toxicity of chlorpyrifos, β-cypermethrin and m...Continue Reading

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Citations

May 5, 2016·Insect Science·Bin ChenYong-Ping Huang
Apr 30, 2017·Insect Biochemistry and Molecular Biology·Fabian SchweizerPhilippe Reymond
May 10, 2019·International Journal of Molecular Sciences·Muhammad HafeezMo Wang
Oct 1, 2017·Nature Ecology & Evolution·Tingcai ChengKazuei Mita
Aug 24, 2020·Pesticide Biochemistry and Physiology·Li XuChengju Wang
Sep 2, 2021·Insect Biochemistry and Molecular Biology·Dries AmezianGaëlle Le Goff

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