Identification of a novel dihydrodaidzein racemase essential for biosynthesis of equol from daidzein in Lactococcus sp. strain 20-92.

Applied and Environmental Microbiology
Yoshikazu ShimadaHaretsugu Hishigaki

Abstract

Equol is metabolized from daidzein, a soy isoflavone, by the gut microflora. In this study, we identified a novel dihydrodaidzein racemase (L-DDRC) that is involved in equol biosynthesis in a lactic acid bacterium, Lactococcus sp. strain 20-92, and confirmed that histidine-tagged recombinant L-DDRC (L-DDRC-His) was able to convert both the (R)- and (S)-enantiomers of dihydrodaidzein to the racemate. Moreover, we showed that recombinant L-DDRC-His was essential for in vitro equol production from daidzein by a recombinant enzyme mixture and that efficient in vitro equol production from daidzein was possible using at least four enzymes, including L-DDRC. We also proposed a model of the metabolic pathway from daidzein to equol in Lactococcus strain 20-92.

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Jan 31, 2012·Journal of Molecular Microbiology and Biotechnology·Yoshikazu ShimadaHaretsugu Hishigaki

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Citations

Sep 21, 2013·Genome Announcements·Hidehiro TohHidetoshi Morita
May 25, 2013·Journal of Clinical Biochemistry and Nutrition·Mariko Uehara
Oct 14, 2014·Food Chemistry·Toshio NiwaToshihiko Osawa
Jun 9, 2016·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Jan F Stevens, Claudia S Maier
Mar 11, 2016·Gut Microbes·Annett Braune, Michael Blaut
Aug 11, 2016·Bioscience of Microbiota, Food and Health·Yuika KawadaTohru Suzuki
Mar 28, 2017·Geriatrics & Gerontology International·Michiya IgaseKatsuhiko Kohara
Mar 6, 2018·Bioscience, Biotechnology, and Biochemistry·Kaeko MurotaMariko Uehara
Apr 27, 2018·Animal Science Journal = Nihon Chikusan Gakkaihō·Hiromi Kimoto-Nira
Jul 23, 2013·International Journal of Food Sciences and Nutrition·Yasuhiro AbiruShigeto Uchiyama
Jun 20, 2018·Frontiers in Microbiology·Hailiang LiYeshi Yin
Sep 6, 2018·FEMS Microbiology Letters·Pyung-Gang LeeByung-Gee Kim
Jul 21, 2021·International Journal of Food Microbiology·Ana Ruiz de la BastidaJosé María Landete
Jun 27, 2021·FEMS Microbiology Letters·Lucía VázquezBaltasar Mayo

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