Identification of a novel family of carbohydrate-binding modules with broad ligand specificity

Scientific Reports
Cheng-Jie DuanJia-Xun Feng

Abstract

Most enzymes that act on carbohydrates include non-catalytic carbohydrate-binding modules (CBMs) that recognize and target carbohydrates. CBMs bring their appended catalytic modules into close proximity with the target substrate and increase the hydrolytic rate of enzymes acting on insoluble substrates. We previously identified a novel CBM (CBMC5614-1) at the C-terminus of endoglucanase C5614-1 from an uncultured microorganism present in buffalo rumen. In the present study, that the functional region of CBMC5614-1 involved in ligand binding was localized to 134 amino acids. Two representative homologs of CBMC5614-1, sharing the same ligand binding profile, targeted a range of β-linked polysaccharides that adopt very different conformations. Targeted substrates included soluble and insoluble cellulose, β-1,3/1,4-mixed linked glucans, xylan, and mannan. Mutagenesis revealed that three conserved aromatic residues (Trp-380, Tyr-411, and Trp-423) play an important role in ligand recognition and targeting. These results suggest that CBMC5614-1 and its homologs form a novel CBM family (CBM72) with a broad ligand-binding specificity. CBM72 members can provide new insight into CBM-ligand interactions and may have potential in protein en...Continue Reading

References

Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·N DinD G Kilburn
Jan 1, 1994·FEMS Microbiology Reviews·P Béguin, J P Aubert
Sep 7, 2000·The Journal of Biological Chemistry·P J SimpsonM P Williamson
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·R P de Vries, J Visser
Mar 9, 2004·The Journal of Biological Chemistry·Joanna L HenshawHarry J Gilbert
Jun 25, 2004·The Biochemical Journal·Alisdair B BorastonGideon J Davies
Oct 27, 2004·The Journal of Biological Chemistry·Alicia Lammerts van BuerenAlisdair B Boraston
Jan 25, 2005·Bioscience, Biotechnology, and Biochemistry·Ehsan AliKazuo Sakka
Oct 20, 2005·Journal of Bioscience and Bioengineering·N HamadaM Okazaki
Nov 29, 2005·Environmental Microbiology·Manuel FerrerPeter N Golyshin
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Oded ShoseyovIlan Levy
Jul 16, 2008·Science in China. Series C, Life Sciences·Lushan WangPeiji Gao
Nov 13, 2009·Applied Microbiology and Biotechnology·Daniel GuillénRomina Rodríguez-Sanoja
May 18, 2010·Applied and Environmental Microbiology·Cheng-Jie DuanJia-Xun Feng
Jul 28, 2010·The Journal of Biological Chemistry·Cedric MontanierHarry J Gilbert
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Cécile HervéJ Paul Knox
Oct 12, 2010·Current Opinion in Biotechnology·Lucía Fernández-ArrojoManuel Ferrer
Apr 2, 2011·The Journal of Biological Chemistry·Nikolaos GeorgelisDaniel J Cosgrove
Jul 14, 2012·Bioscience, Biotechnology, and Biochemistry·Nhung Hong NguyenSomchai Pongpattanakitshote
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nikolaos GeorgelisDaniel J Cosgrove
Dec 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Fiona CuskinHarry J Gilbert
Dec 12, 2012·The Journal of Biological Chemistry·Ana S LuísHarry J Gilbert
Jun 19, 2013·Current Opinion in Structural Biology·Harry J GilbertAlisdair B Boraston
Nov 26, 2013·Nucleic Acids Research·Vincent LombardBernard Henrissat

❮ Previous
Next ❯

Citations

May 30, 2017·Applied Microbiology and Biotechnology·Cheng-Jie DuanJia-Xun Feng
Jun 1, 2018·BMC Evolutionary Biology·Siddhartha Kundu, Rita Sharma
Mar 5, 2021·Archives of Biochemistry and Biophysics·Yousra El MannaiEiji Suzuki
Mar 21, 2021·Insect Biochemistry and Molecular Biology·Abhishek Ojha, Wenqing Zhang
Mar 22, 2021·Journal of Structural Biology·Dario HeymannAli Miserez
Aug 27, 2021·Applied Microbiology and Biotechnology·Pattsarun CheawchanlertfaChakrit Tachaapaikoon
Dec 18, 2021·Development, Growth & Differentiation·Toshihiro AraeMisato Ohtani

❮ Previous
Next ❯

Datasets Mentioned

BETA
ACA61140
EU449484
CAJ19146

Methods Mentioned

BETA
electrophoresis
PCR
protein assay

Software Mentioned

CDROM Biokine
BLAST
ClustalW
DNAStar
Origin
GraphPad
Graphpad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.