Identification of a Novel Function of Adipocyte Plasma Membrane-Associated Protein (APMAP) in Gestational Diabetes Mellitus by Proteomic Analysis of Omental Adipose Tissue

Journal of Proteome Research
Yuhang MaYongde Peng

Abstract

Gestational diabetes mellitus (GDM) is considered as an early stage of type 2 diabetes mellitus. In this study, we compared demographic and clinical data between six GDM subjects and six normal glucose tolerance (NGT; healthy controls) subjects and found that homeostasis model of assessment for insulin resistance index (HOMA-IR) increased in GDM. Many previous studies demonstrated that omental adipose tissue dysfunction could induce insulin resistance. Thus, to investigate the cause of insulin resistance in GDM, we used label-free proteomics to identify differentially expressed proteins in omental adipose tissues from GDM and NGT subjects (data are available via ProteomeXchange with identifier PXD003095). A total of 3528 proteins were identified, including 66 significantly changed proteins. Adipocyte plasma membrane-associated protein (APMAP, a.k.a. C20orf3), one of the differentially expressed proteins, was down-regulated in GDM omental adipose tissues. Furthermore, mature 3T3-L1 adipocytes were used to simulate omental adipocytes. The inhibition of APMAP expression by RNAi impaired insulin signaling and activated NFκB signaling in these adipocytes. Our study revealed that the down-regulation of APMAP in omental adipose tissue...Continue Reading

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Citations

Dec 29, 2017·Analytical and Bioanalytical Chemistry·Peter E FeistAmanda B Hummon
Jun 19, 2018·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Yuhang MaYufan Wang
Jul 30, 2020·Journal of Diabetes Research·Tao ZhouShi-Wen Jiang
Jun 17, 2021·European Journal of Clinical Nutrition·Cara TrivettDilys J Freeman

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