Identification of a novel hybrid plasmid coproducing MCR-1 and MCR-3 variant from an Escherichia coli strain

The Journal of Antimicrobial Chemotherapy
Ruichao LiLi Bai

Abstract

To characterize the genome of an Escherichia coli harbouring both mcr-1 and mcr-3.19 on a hybrid plasmid and the underlying transmission mechanisms. Broth microdilution was used to perform antimicrobial susceptibility testing. Conjugation assays and S1-PFGE were used to assess the transferability of mcr genes. Resistance genotypes and genetic contexts were investigated, based on WGS data from the Illumina and MinION platforms. Inverse PCR was performed to test the mcr-3.19-bearing circular intermediate. Bioinformatic tools were used to further characterize the hybrid plasmid. E. coli CP53 was identified as harbouring both mcr-1 and mcr-3.19 on a 231 859 bp hybrid plasmid pCP53-mcr1_3 containing IncFIA, IncHI1A, IncHI1B and IncN replicons. The genetic structures of mcr-1 and mcr-3.19 were similar to those reported in other mcr-1 and mcr-3.19-bearing plasmids, which suggested that recombination between mcr-bearing plasmids had been mediated by ISs. However, the MDR plasmid pCP53-mcr1_3 cannot transfer via conjugation. Furthermore, another three plasmids were identified in the isolate, two of which encoded resistance genes. In640 duplication between two MDR plasmids was observed. An MDR-region recombination existed in E. coli CP53...Continue Reading

References

Aug 12, 2017·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Marta HernándezAlberto Quesada
Feb 21, 2018·The Journal of Antimicrobial Chemotherapy·Jiyun LiCongming Wu
Jun 19, 2018·The Journal of Antimicrobial Chemotherapy·Yong-Qiang YangHong-Ning Wang
Jul 5, 2018·Emerging Microbes & Infections·Xiaoming WangYang Wang

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Citations

Jun 19, 2019·Antimicrobial Agents and Chemotherapy·Pei ZhangRuichao Li
Mar 3, 2020·Environmental Microbiology·Yingbo ShenYang Wang
Oct 30, 2020·Infection and Drug Resistance·Jiayang LiuJiazhang Qiu

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