PMID: 2499310Mar 15, 1989Paper

Identification of a novel inflammatory stimulant of chondrocytes. Early events in cell activation by bradykinin receptors on pig articular chondrocytes

The Biochemical Journal
H P BentonM R Hanley

Abstract

The inflammatory peptide bradykinin stimulated a rapid and transient increase in cytoplasmic [Ca2+] in primary pig chondrocytes, as measured by the fluorescent indicator dye Fura-2. This increase occurred in the absence of extracellular Ca2+, indicating a mobilization from intracellular stores. The elevation in intracellular [Ca2+] was mediated by authentic bradykinin receptors, since it was blocked by the specific bradykinin antagonist [beta-(2-thienyl)-L-Ala5,8,D-Phe7]bradykinin. Activation of chondrocytes by bradykinin induced a concentration-dependent [ED50 (dose for half-maximal response) approximately 40 nM] accumulation of inositol monophosphate in the presence of LiCl and a concentration-dependent increase in production of prostaglandin E2. The generation of the secondary mediator prostaglandin E2 was a biologically relevant output response induced by bradykinin, but chondrocyte responses, such as the rate of entry into DNA synthesis, the rate and pattern of new protein synthesis and the rate of synthesis and resorption of cartilage proteoglycan, were unaltered by bradykinin treatment. Chondrocytes were also shown to be activated by two pharmacological mediators of cytosolic [Ca2+] elevation, i.e. the ionophore A23187 a...Continue Reading

Citations

Dec 1, 1994·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·J N Sharma, W W Buchanan
Oct 16, 2010·British Journal of Pharmacology·S MeiniC A Maggi
Jun 1, 1993·British Journal of Pharmacology·U KachintornK E Barrett
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Jun 11, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M KoolpeH P Benton
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Sep 14, 2001·Psychology and Aging·P A AllenM D Murphy
Oct 12, 2001·The Journal of Steroid Biochemistry and Molecular Biology·V L SylviaZ Schwartz

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