Identification of a novel isoform of mouse dentin matrix protein 1: spatial expression in mineralized tissues

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Mary MacDougallJ Chen

Abstract

Dentin matrix protein 1 (Dmp1) is an acidic phosphoprotein first identified by cDNA cloning from a rat tooth library. Northern blot hybridization of a variety of tissues detected Dmp1 mRNAs only in odontoblasts, suggesting that this protein was odontoblast specific. In situ hybridization studies showed expression of Dmp1 in odontoblasts with transient expression in secretory ameloblasts. The purpose of this study was to isolate and characterize a mouse Dmp1 cDNA and determine its spatial expression pattern related to other mineralizing tissues. A mouse molar cDNA library was screened with a 32P-labeled Dmp1 polymerase chain reaction amplification product in order to isolate a full-length clone. DNA sequence analysis of the largest mouse Dmp1 cDNA (2802 base pairs [bp]) revealed an open reading frame of 1509 nucleotides encoding a 503 amino acid protein with a single polyadenylation signal. Comparison with rat and bovine Dmp1 sequence showed high homology and the identification of a 45 bp (15 amino acid) insert, representing an alternative spliced mRNA. This 45 bp segment was shown to represent a small exon by DNA analysis of a mouse genomic Dmp1 clone. In situ hybridization studies revealed a much broader Dmp1 tissue expression...Continue Reading

References

Dec 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A GeorgeA Veis
Aug 1, 1993·Human Molecular Genetics·R D GooldS B Masters
Feb 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A H CrosbyM J Dixon
Jan 1, 1996·Cytogenetics and Cell Genetics·M MacDougallR J Leach
Jan 1, 1996·Connective Tissue Research·M MacDougallD Simmons
Mar 1, 1997·Journal of Dental Research·K L HirstM J Dixon

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Citations

May 20, 2008·Calcified Tissue International·Bingzhen HuangChunlin Qin
Jun 25, 2008·Journal of Dental Research·L YeJ Q Feng
Aug 14, 2008·Cells, Tissues, Organs·Izabela MaciejewskaChunlin Qin
Aug 30, 2008·Cells, Tissues, Organs·Yongbo LuJian Q Feng
Sep 2, 2008·The Journal of Biological Chemistry·Sriram RavindranAnne George
Nov 13, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jianjun HaoAnne George
Aug 4, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Yao SunChunlin Qin
May 11, 2011·Cells, Tissues, Organs·Yao SunChunlin Qin
Jun 11, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Suzanne M Jan De BeurSusan C Schiavi
Nov 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S ToyosawaT Komori
Oct 9, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jian Q FenMary MacDougall
May 9, 2012·Development Genes and Evolution·Caterina CostaValeria Matranga
May 11, 2012·Biomedical Research·Angela Quispe-SalcedoHayato Ohshima
Mar 1, 2006·Archives of Histology and Cytology·Hironobu SuzukiTakeyasu Maeda
Jan 31, 2013·Journal of Molecular Evolution·Jérémie SilventSidney Delgado
Jun 16, 2012·The Journal of Endocrinology·Katherine A StainesColin Farquharson
Sep 4, 2003·Connective Tissue Research·Mary MacDougall
Aug 27, 2014·Connective Tissue Research·Alexander JacobAnne George
Jan 19, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Kostas VerdelisElia Beniash
May 6, 2006·International Endodontic Journal·C MesgouezA Berdal
Oct 6, 2007·International Endodontic Journal·M J HondaM Ueda
Mar 21, 2008·European Journal of Oral Sciences·Bingzhen HuangChunlin Qin
Feb 3, 2007·Archives of Oral Biology·J Gluhak-HeinrichS E Harris
Jun 10, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C QinW T Butler
Apr 21, 2005·Journal of Dental Research·D D Bosshardt
Feb 4, 2010·Brazilian Dental Journal·Elizabeth Ferreira MartinezVera Cavalcanti de Araújo
Sep 2, 2006·Stem Cells·Hidetoshi YamazakiShin-Ichi Hayashi
Jan 20, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·F LézotA Berdal
Feb 20, 2015·Journal of Dental Research·J D PadovanoA George
Aug 16, 2015·Journal of Dental Research·L WangM J Somerman

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