Abstract
RUNX1 gene, a master regulator of the hematopoietic process, participates in pathological conditions as a partner for several genes in chromosomal translocations. One of the most frequent chromosomal translocations found in acute myeloid leukemia patients is the t(8;21), in which RUNX1 and ETO genes recombine. In RUNX1 gene, the DNA double-strand breaks that originate the t(8;21) are generated in the intron 5, specifically within three regions designated as BCR1, BCR2, and BCR3. To date, what determines that these regions are more susceptible to DNA double-strand breaks is not completely clear. In this report, we characterized RUNX1 intron 5, by analyzing DNase-seq and ChIP-seq data, available in the ENCODE Project server, to evaluate DNaseI hypersensitivity and the presence of the epigenetic mark H3K4me3 in 124 and 51 cell types, respectively. Our results show that intron 5 exhibits an epigenetic mark distribution similar to known promoter regions. Moreover, using the online tool YAPP and available CAGE data from the ENCODE Project server, we identified several putative transcription start sites within intron 5 in regions BCR2 and BCR3. Finally, available EST data was analyzed, identifying a novel uncharacterized long non-codi...Continue Reading
References
Jun 1, 1995·Genes & Development·T Kiss, W Filipowicz
Aug 1, 1994·Leukemia & Lymphoma·G Nucifora, J D Rowley
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y YuanD E Zhang
Oct 19, 2001·Oncogene·J D Licht
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yanming ZhangJanet D Rowley
Aug 18, 2006·Molecular and Cellular Biology·Chunhong Yan, Douglas D Boyd
Mar 14, 2007·Blood·Lori A WagnerGerald J Gleich
Aug 5, 2008·Genes & Development·Alexander K EbralidzeDaniel G Tenen
Oct 15, 2008·Journal of Cellular Physiology·Marcela StuardoSoraya E Gutiérrez
Jan 9, 2009·Nature·Michael J ChenNancy A Speck
Jan 16, 2009·Blood·Xueqing ZhangPeter E Newburger
May 29, 2009·Leukemia·Y ChengJ Jin
Nov 13, 2010·Nucleic Acids Research·David RearickAlexei Fedorov
Dec 23, 2010·Future Oncology·Etienne De BraekeleerMarc De Braekeleer
Sep 8, 2012·Genome Research·Thomas DerrienRoderic Guigó
Nov 8, 2013·Immune Network·Yeonsook MoonGyeongsin Park
Apr 23, 2014·International Journal of Cancer. Journal International Du Cancer·Hong WangJi-Fan Hu
Aug 6, 2014·Nucleic Acids Research·Jingnan SunJi-Fan Hu
Dec 3, 2014·Experimental Cell Research·C Drevon, T Jaffredo
Oct 26, 2016·Oncology Reports·Ioannis PanagopoulosSverre Heim
Mar 28, 2017·Scientific Reports·Qianqian NingGuangjun Yu
Jul 20, 2017·Journal of Translational Internal Medicine·Ming Hong, Guangsheng He
Aug 16, 2017·Seminars in Cell & Developmental Biology·Vincent BoivinMichelle S Scott
Oct 14, 2017·Cancer Genetics·Nicole FoleyGabriel N Mannis
Apr 15, 2018·Leukemia·Phuong Cao Thi NgocTakaomi Sanda
Apr 29, 2018·Molecular Cancer·Joëlle El HajjEvelyne Ségal-Bendirdjian
Dec 1, 2018·Cell Biochemistry and Function·Jinxin ShiZhenning Wang