Identification of a novel mechanism of action of bovine IgG antibodies specific for Staphylococcus aureus

Veterinary Research
Mutsumi FurukawaHisashi Aso

Abstract

Staphylococcus aureus is a major pathogen that causes subclinical mastitis associated with huge economic losses to the dairy industry. A few vaccines for bovine mastitis are available, and they are expected to induce the production of S. aureus-specific antibodies that prevent bacterial adherence to host cells or promote opsonization by phagocytes. However, the efficacy of such vaccines are still under debate; therefore, further research focusing on improving the current vaccines by seeking additional mechanisms of action is required to reduce economic losses due to mastitis in the dairy industry. Here, we generated S. aureus-specific bovine IgG antibodies (anti-S. aureus) that directly inhibited bacterial growth in vitro. Inhibition depended on specificity for anti-S. aureus, not the interaction between Protein A and the fragment crystallizable region of the IgG antibodies or bacterial agglutination. An in vitro culture study using S. aureus strain JE2 and its deletion mutant JE2ΔSrtA, which lacks the gene encoding sortase A, revealed that the effect of anti-S. aureus was sortase-A-independent. Sortase A is involved in the synthesis of cell-wall-associated proteins. Thus, other surface molecules, such as membrane proteins, cel...Continue Reading

References

Oct 12, 2000·International Journal of Food Microbiology·N Balaban, A Rasooly
Feb 24, 2001·International Journal of Systematic and Evolutionary Microbiology·D Fritze, R Pukall
Dec 5, 2002·Journal of Mammary Gland Biology and Neoplasia·Lorraine M Sordillo, Katie L Streicher
Jan 1, 1963·Journal of Bacteriology·D JONESC F NIVEN
Jul 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Koichiro TamuraSudhir Kumar
Oct 26, 2005·Transgenic Research·David M DonovanRobert J Wall
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Luciano A MarraffiniOlaf Schneewind
Nov 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yukiko K Stranger-JonesOlaf Schneewind
Nov 24, 2006·Journal of the South African Veterinary Association·K R PetrovskiG Buneski
Nov 21, 2008·Letters in Applied Microbiology·M De KwaadstenietL M T Dicks
Aug 28, 2012·Human Vaccines & Immunotherapeutics·Annaliesa S AndersonKathrin U Jansen
Aug 27, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Vincent Peton, Yves Le Loir
Oct 3, 2013·MBio·Scott D Kobayashi, Frank R DeLeo
Jul 6, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·John J VarroneEdward M Schwarz
Jul 8, 2014·Nature Reviews. Microbiology·Wilmara Salgado-Pabón, Patrick M Schlievert
Jan 15, 2015·Current Microbiology·Jakub KwiecinskiTao Jin
May 16, 2015·Research in Veterinary Science·F ScaliA Zecconi
Jul 15, 2015·Veterinary Research·Satoru TakanashiHisashi Aso
Nov 26, 2015·Science Advances·Cindy M LiuPaal Skytt Andersen
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Apr 28, 2016·Trends in Microbiology·Sumitra D MitraKimberly A Kline
Aug 12, 2016·Microbiology and Molecular Biology Reviews : MMBR·Minh Thu Nguyen, Friedrich Götz
Dec 6, 2016·Advanced Drug Delivery Reviews·Zehra ElgundiVeysel Kayser

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Citations

Aug 2, 2020·Journal of Applied Microbiology·Z ShakeranR Emamzadeh

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Methods Mentioned

BETA
Protein Assay
ELISA
scanning electron microscopy

Software Mentioned

Prism
MEGA7
STARTVAC
FlowJo
GraphPad

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