Identification of a novel metallo-β-lactamase, CAM-1, in clinical Pseudomonas aeruginosa isolates from Canada

The Journal of Antimicrobial Chemotherapy
David A BoydMichael R Mulvey

Abstract

To identify the β-lactamase responsible for the positive detection of carbapenemase production in four clinical isolates of Pseudomonas aeruginosa that were negative by PCR for KPC, OXA-48, NDM, VIM, IMP, GES and NMC/IMI carbapenemase genes. WGS using short-read and long-read methods was used to characterize the isolates. Bioinformatic analysis was used to identify the potential gene encoding a carbapenemase. Cloning, antimicrobial susceptibility testing and biochemical and phenotypic characterization were used to determine metallo-enzyme activity. Single-nucleotide variant (SNV) typing was used to determine strain relatedness. Conjugation experiments were used to determine transmissibility of the novel carbapenemase-encoding gene. WGS analysis revealed a novel class B β-lactamase gene, blaCAM-1 (Central Alberta Metallo-β-lactamase), located in a 73 kb integrative element, named IMEPaCAM-1, in the chromosome of four clinical isolates of P. aeruginosa. The cloned blaCAM-1 gene conferred carbapenem resistance to Escherichia coli TOP10. The four isolates, which were all closely related, were from three patients, all of whom spent time in the same hospital in 2008 and/or 2009. IMEPaCAM-1 could not be transferred by conjugation. A n...Continue Reading

References

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Citations

Apr 19, 2019·Journal of Clinical Microbiology·Romney M Humphries
Jul 22, 2020·Antimicrobial Agents and Chemotherapy·Sara E BoydWilliam W Hope
Oct 3, 2020·The Journal of Antimicrobial Chemotherapy·Fanny BerglundErik Kristiansson
Mar 9, 2021·Frontiers in Microbiology·Eun-Jeong Yoon, Seok Hoon Jeong

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