Identification of a novel mutation affecting domain V of the 23S rRNA gene in Helicobacter pylori

Helicobacter
S ToracchioLeonardo Marzio

Abstract

This study analyzes clarithromycin resistance status and 23S rRNA gene mutations in Helicobacter pylori strains from Central Italian patients. H. pylori strains from 235 dyspeptic patients (205 with no history of clarithromycin exposure and 30 referred for failure of eradication therapy) were tested for clarithromycin resistance by screening agar method and E-test. Resistant strains were analyzed for mutations of the 23S rRNA gene by PCR-RFLP and sequencing. Primary resistance was observed in strains from 43/205 (21%) patients with no history of clarithromycin exposure and secondary resistance in 30/30 (100%) strains from previously treated patients. A single mutant strain was detected in 54/73 (74%) cases, a mixture of one or more mutant(s) plus the wild type in the remaining 19/73 (26%) cases. One 23S rRNA gene mutation (A-->T transversion at nucleotide 2144) in the peptidyltransferase region of domain V was novel. This study shows: (a) a high prevalence of H. pylori strains with primary or secondary clarithromycin resistance in an urban area of Central Italy; (b) colonization by both mutant and wild-type H. pylori in the same patient; (c) a novel variant of the H. pylori 23S rRNA gene.

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Citations

Feb 5, 2011·Nature Reviews. Gastroenterology & Hepatology·Emiko RimbaraDavid Y Graham
Mar 29, 2005·Antimicrobial Agents and Chemotherapy·Jong Hwa LeeSang Hee Lee
Jul 4, 2009·World Journal of Gastroenterology : WJG·Norazah AhmadRamelah Mohamed
Jun 26, 2007·International Journal of Antimicrobial Agents·Emiko RimbaraMasanori Sasatsu
Mar 23, 2010·Helicobacter·Josette RaymondChristophe Burucoa
Dec 18, 2013·Journal of Medical Microbiology·Vincenzo De FrancescoDino Vaira
Sep 12, 2015·Journal of Chemotherapy·Doaa GhaithIman Ramzy
Oct 28, 2006·The Lancet Infectious Diseases·Monique M GerritsJohannes G Kusters
May 20, 2017·World Journal of Gastroenterology : WJG·Nazlı ArslanEbru Demiray-Gürbüz
Feb 1, 2017·The Journal of Pathology. Clinical Research·Ebru Demiray-GürbüzGuillermo I Perez Perez
Apr 18, 2018·World Journal of Gastroenterology : WJG·Andrés Jenuer MattaAlvaro Jairo Pazos

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