Identification of a novel nifH-like (frxC) protein in chloroplasts of the liverwort Marchantia polymorpha

Plant Molecular Biology
Y FujitaH Matsubara

Abstract

The frxC gene, one of the unidentified open reading frames present in liverwort chloroplast DNA, shows significant homology with the nifH genes coding for the Fe protein, a component of the nitrogenase complex (Ohyama et al., 1986, Nature 322: 572-574). A truncated form of the frxC gene was designed to be over-expressed in Escherichia coli and an antibody against this protein was prepared using the purified product as an antigen. This antibody reacted with a protein in the soluble fraction of liverwort chloroplasts, which had an apparent molecular weight of 31,000, as revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, in good agreement with a putative molecular weight of 31,945 deduced from the DNA sequence of the frxC gene. In a competitive inhibition experiment, the antigenicity of this protein was indicated to be similar to that of the over-expressed protein in E. coli. Therefore, we concluded that the frxC gene was expressed in liverwort chloroplasts and that its product existed in a soluble form. The molecular weight of the frxC protein was approximately 67,000, as estimated by gel filtration chromatography, indicating that the frxC protein may exist as a dimer of two identical polypeptides analogous to...Continue Reading

References

Jan 1, 1979·Annual Review of Biochemistry·L E Mortenson, R N Thorneley
Jan 1, 1988·Trends in Biochemical Sciences·K OhyamaY Yamada
Sep 12, 1988·Nucleic Acids Research·R Jones, R Haselkorn
Jan 1, 1985·Annual Review of Biophysics and Biophysical Chemistry·W H Orme-Johnson
Jan 1, 1972·Methods in Enzymology·R H Burris
Jan 1, 1970·Archiv für Mikrobiologie·W D Stewart, M Lex
Jan 1, 1981·Cold Spring Harbor Symposia on Quantitative Biology·F M Ausubel, F C Cannon
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M F CharetteA Markovitz
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C H Chung, A L Goldberg
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M MevarechR Haselkorn

❮ Previous
Next ❯

Citations

Nov 1, 1995·Photosynthesis Research·H MatsubaraY Fujita
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D H BurkeA Sidow
Jan 1, 1991·Plant Physiology·Y TakahashiH Matsubara
Apr 15, 1996·European Journal of Biochemistry·S Reinbothe, C Reinbothe
Jan 28, 2004·Photochemistry and Photobiology·Benoît Schoefs, Fabrice Franck
Oct 7, 2018·Protoplasma·Pratishtha Vedalankar, Baishnab C Tripathy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.