Identification of a novel NPR1-like gene from Nicotiana glutinosa and its role in resistance to fungal, bacterial and viral pathogens

Plant Biology
Y ZhangX-Q Guo

Abstract

The NPR1 or NPR1-like genes play a pivotal role in systemic acquired resistance in plants. Here, we isolated and identified a novel tobacco (Nicotiana glutinosa) NPR1-like gene (designated as NgNPR3). The full-length cDNA is 2049 bp in length with a 1767 bp open reading frame which encodes a 588 amino acids protein with an estimated molecular mass of 66 kDa and a calculated pI of 7.14. Homology analysis suggested that the NgNPR3 protein shares significant similarity to AtNPR3 of Arabidopsis. Transient expression assay of NgNPR3-GFP fusion gene in onion epidermal cells revealed that the NgNPR3 protein was localized to the cytoplasm and moved into the nucleus after redox change. RT-PCR results indicated that NgNPR3 was up-regulated after treatment with SA, INA, H(2)O(2,) and MeJA, which play important roles in various resistance responses in tobacco. Transcriptional level of NgNPR3 was also up-regulated after inoculation with Rhizoctonia solani, Phytophthora parasitica, Alternaria alternata, Pseudomonas solanacearum, and potato virus Y (PVY), respectively. When NgNPR3 was overexpressed in N. tabacum cv. Samsun plants, the transgenic plants showed enhanced resistance to the pathogens A. alternate, P. solanacearum and PVY. Furtherm...Continue Reading

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Citations

May 19, 2012·Journal of Plant Physiology·María José Clemente-MorenoJosé Antonio Hernández
Mar 28, 2018·Horticulture Research·Katchen Julliany P SilvaKevin M Folta
Feb 4, 2011·Proceedings. Biological Sciences·Sam C BanksMichaela D J Blyton

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