Identification of a Novel Plasmid-Mediated Carbapenemase-Encoding Gene, blaVMB-2, in Vibrio diabolicus.

Antimicrobial Agents and Chemotherapy
Ming LiuRuichao Li

Abstract

Here, we characterized a carbapenem-resistant Vibrio diabolicus strain of shrimp origin with various experiments and bioinformatics analysis. A novel metallo-β-lactamase (MBL) gene, blaVMB-2, that confers resistance to β-lactams, including meropenem and cephalosporins, was identified on a plasmid-borne composite transposon, ISShfr9-ISCR1-blaVMB-2-blaCARB-12-aadA1-ISShfr9, which is capable of generating a blaVMB-2-bearing circular intermediate. ISShfr9 was found to be disseminated in multidrug-resistant (MDR) pathogens, arousing the concern of further transmission of a blaVMB-2-bearing circular intermediate to clinical Enterobacterales strains via such insertion sequences, which warrants further investigations.

References

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Datasets Mentioned

BETA
CP069197
CP069194
MN865127
CP020079

Methods Mentioned

BETA
PCR

Software Mentioned

FastTree
RAST
BRIG
BLASTn
ISfinder
ResFinder
Roary
Unicycler

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