Identification of a Novel Small Cysteine-Rich Protein in the Fraction from the Biocontrol Fusarium oxysporum Strain CS-20 that Mitigates Fusarium Wilt Symptoms and Triggers Defense Responses in Tomato

Frontiers in Plant Science
Larisa A ShcherbakovaSergey K Zavriev

Abstract

The biocontrol effect of the non-pathogenic Fusarium oxysporum strain CS-20 against the tomato wilt pathogen F. oxysporum f. sp. lycopersici (FOL) has been previously reported to be primarily plant-mediated. This study shows that CS-20 produces proteins, which elicit defense responses in tomato plants. Three protein-containing fractions were isolated from CS-20 biomass using size exclusion chromatography. Exposure of seedling roots to one of these fractions prior to inoculation with pathogenic FOL strains significantly reduced wilt severity. This fraction initiated an ion exchange response in cultured tomato cells resulting in a reversible alteration of extracellular pH; increased tomato chitinase activity, and induced systemic resistance by enhancing PR-1 expression in tomato leaves. Two other protein fractions were inactive in seedling protection. The main polypeptide (designated CS20EP), which was specifically present in the defense-inducing fraction and was not detected in inactive protein fractions, was identified. The nucleotide sequence encoding this protein was determined, and its complete amino acid sequence was deduced from direct Edman degradation (25 N-terminal amino acid residues) and DNA sequencing. The CS20EP was...Continue Reading

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Citations

Nov 9, 2018·Frontiers in Bioengineering and Biotechnology·Gustavo Pagotto BorinJuliana Velasco de Castro Oliveira
Mar 3, 2020·Frontiers in Plant Science·Francisco J de Lamo, Frank L W Takken

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Datasets Mentioned

BETA
KR028481

Methods Mentioned

BETA
transgenic
Gel Filtration
Size-Exclusion
size-exclusion chromatography
electrophoresis
PCR

Software Mentioned

Oligo
MEGA5
BLAST
Evrogen
ImageJ
STATISTICA

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