PMID: 12960322Sep 10, 2003Paper

Identification of a novel subpopulation of human cord blood CD34-CD133-CD7-CD45+lineage- cells capable of lymphoid/NK cell differentiation after in vitro exposure to IL-15

The Journal of Immunology : Official Journal of the American Association of Immunologists
Sergio RutellaLuca Pierelli

Abstract

The hemopoietic stem cell (HSC) compartment encompasses cell subsets with heterogeneous proliferative and developmental potential. Numerous CD34(-) cell subsets that might reside at an earlier stage of differentiation than CD34(+) HSCs have been described and characterized within human umbilical cord blood (UCB). We identified a novel subpopulation of CD34(-)CD133(-)CD7(-)CD45(dim)lineage (lin)(-) HSCs contained within human UCB that were endowed with low but measurable extended long-term culture-initiating cell activity. Exposure of CD34(-)CD133(-)CD7(-)CD45(dim)lin(-) HSCs to stem cell factor preserved cell viability and was associated with the following: 1) concordant expression of the stem cell-associated Ags CD34 and CD133, 2) generation of CFU-granulocyte-macrophage, burst-forming unit erythroid, and megakaryocytic aggregates, 3) significant extended long-term culture-initiating cell activity, and 4) up-regulation of mRNA signals for myeloperoxidase. At variance with CD34(+)lin(-) cells, CD34(-)CD133(-)CD7(-)CD45(dim)lin(-) HSCs maintained with IL-15, but not with IL-2 or IL-7, proliferated vigorously and differentiated into a homogeneous population of CD7(+)CD45(bright)CD25(+)CD44(+) lymphoid progenitors with high expres...Continue Reading

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Citations

Jul 14, 2007·Transplantation·Atsuhiko OikawaNobukata Shinohara
Oct 3, 2009·British Journal of Haematology·Michael R Verneris, Jeffrey S Miller
Jun 24, 2017·Frontiers in Immunology·Anne M DickinsonHans Jochem-Kolb
May 26, 2009·São Paulo Medical Journal = Revista Paulista De Medicina·Jerusa Martins CarvalhoJosé Salvador Rodrigues de Oliveira
Aug 1, 2013·Targeted Oncology·Nate N WaldronDaniel A Vallera
Feb 24, 2017·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Jörg U SchmohlDaniel A Vallera
May 31, 2016·Toxins·Jörg U Schmohl, Daniel A Vallera
Jun 2, 2015·Frontiers in Immunology·Gianfranco PittariSergio Rutella
Nov 21, 2009·Circulation Journal : Official Journal of the Japanese Circulation Society·Bernhard SchlechtaAlfred A Kocher
Apr 8, 2017·Frontiers in Immunology·Anushruti SarvariaAurore Saudemont
Jan 19, 2016·Frontiers in Medicine·Rohtesh S MehtaKatayoun Rezvani
Jun 11, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sergio RutellaGabriella Ferrandina
Sep 4, 2020·Hematology/oncology and Stem Cell Therapy·Uroosa Ibrahim, Alla Keyzner
May 29, 2020·Cell Stem Cell·Parvez VoraSheila Singh

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