Identification of a novel tetramerization domain in large conductance K(ca) channels

Neuron
J C Quirk, P H Reinhart

Abstract

More than 50 genes are known to encode K(+) channel monomers and can coassemble to form hetero-tetrameric K(+) channels. However, only a subset of possible monomer combinations come together to form functional ion channels. The assembly and tetramerization of appropriate channel monomers is mediated by association domains (ADs). To identify such domains in human large-conductance Ca(2+)-activated K(+) channels (hSlo1), we screened hSlo1 domains for self-association using yeast two-hybrid assays. Putative ADs were subjected to functional assays in Xenopus oocytes and further characterized by coprecipitation, native gel electrophoresis, and sucrose density gradient centrifugation assays. This led to the identification of a single intracellular association domain localized near the channel pore and required for channel function. We conclude that this novel tetramerization domain, referred to as BK-T1, promotes the assembly of hSlo1 monomers into functional K(Ca) channels.

References

Jan 1, 1989·Neuron·P H ReinhartI B Levitan
Dec 1, 1984·The Journal of Physiology·R Miledi, I Parker
Aug 22, 1995·Proceedings. Biological Sciences·E GlowatzkiB Fakler
Jun 1, 1996·Current Opinion in Neurobiology·J Roeper, O Pongs
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·M WallnerL Toro
Jan 1, 1997·Annual Review of Neuroscience·L Y Jan, Y N Jan
Sep 1, 1997·Biophysical Journal·M Schreiber, L Salkoff
Mar 7, 1998·The Journal of Biological Chemistry·M SchreiberL Salkoff
Aug 4, 1998·Current Opinion in Neurobiology·C VergaraJ P Adelman
Aug 26, 1998·Methods in Enzymology·Z Sheng, C Deutsch
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·P J MylerK Stuart
Mar 30, 1999·Biophysical Journal·L Tu, C Deutsch
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·M WallnerL Toro
Apr 1, 1999·Annual Review of Physiology·R Fettiplace, P A Fuchs
May 13, 1999·Nature Neuroscience·M SchreiberL Salkoff
Jul 13, 1999·Neuron·D M Papazian
Jul 22, 1999·Annals of the New York Academy of Sciences·W A CoetzeeB Rudy
Jul 22, 1999·Annals of the New York Academy of Sciences·C T BondJ P Adelman
Jul 27, 1999·Current Opinion in Chemical Biology·G J Kaczorowski, M L Garcia
Mar 16, 2000·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T NagaseO Ohara
Jun 6, 2000·Trends in Neurosciences·R A North

❮ Previous
Next ❯

Citations

Dec 23, 2008·Cellular and Molecular Life Sciences : CMLS·J CuiU S Lee
Oct 13, 2010·Pflügers Archiv : European journal of physiology·Asser Nyander PoulsenDan Arne Klaerke
Jun 2, 2007·Journal of the Association for Research in Otolaryngology : JARO·Irene S GabashviliAnne B S Giersch
Jan 20, 2004·Trends in Biochemical Sciences·Tarmo P RoosildSenyon Choe
Sep 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Gonzalo BudelliLawrence Salkoff
Nov 2, 2005·The Journal of General Physiology·Teresa GiraldezFred J Sigworth
Oct 7, 2004·Science's STKE : Signal Transduction Knowledge Environment·Frank H Yu, William A Catterall
May 9, 2008·American Journal of Physiology. Renal Physiology·Daniel I LevySteve A N Goldstein
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·John P MorrowSteven O Marx
Oct 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sang-Ho Kwon, William B Guggino
Jul 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lijun TianMichael J Shipston
Nov 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Claire E McCartneyIain C M Rowe
Apr 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Haining ZhongKing-Wai Yau
Jul 7, 2010·The Journal of Physiology·Ramon LatorreCristian Zaelzer
Apr 3, 2010·Progress in Neurobiology·Kate L WeatherallNeil V Marrion
Feb 9, 2008·Biochimica Et Biophysica Acta·Jiuyong Xie
Nov 20, 2008·The FEBS Journal·Zhiguang YuchiDaniel S C Yang
Sep 13, 2013·Channels·Gustavo F ContrerasRamón Latorre
Apr 21, 2006·Biochimica Et Biophysica Acta·Daisy SahooMargery A Connelly
Nov 11, 2008·Biochimica Et Biophysica Acta·Asser Nyander PoulsenDan A Klaerke
Dec 17, 2005·Pharmacology & Therapeutics·Srinivas GhattaStephen T O'Rourke
Dec 6, 2002·Nature·Rebecca Piskorowski, Richard W Aldrich
Jul 18, 2003·Journal of Neurophysiology·Manuel KukuljanAngeles B Ribera
Oct 25, 2006·Molecular Membrane Biology·Heidi De WetRichard Callaghan
Nov 29, 2002·American Journal of Physiology. Cell Physiology·Angelos-Aristeidis KonstasStephen J Tucker
Jan 2, 2003·The Journal of Biological Chemistry·Lijun TianMichael J Shipston
Nov 4, 2016·Physiological Reviews·Ramon LatorreOsvaldo Alvarez
Jul 24, 2002·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Patricio OrioRamón Latorre
Nov 23, 2016·Proceedings of the National Academy of Sciences of the United States of America·Pablo MirandaMiguel Holmgren

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.