Identification of a novel trimeric autotransporter adhesin in the cryptic genospecies of Haemophilus.

Journal of Bacteriology
Amanda J SheetsJ W St Geme

Abstract

Haemophilus biotype IV strains belonging to the recently recognized Haemophilus cryptic genospecies are an important cause of maternal genital tract and neonatal systemic infections and initiate infection by colonizing the genital or respiratory epithelium. To gain insight into the mechanism of Haemophilus cryptic genospecies colonization, we began by examining prototype strain 1595 and three other strains for adherence to genital and respiratory epithelial cell lines. Strain 1595 and two of the three other strains demonstrated efficient adherence to all of the cell lines tested. With a stably adherent variant of strain 1595, we generated a Mariner transposon library and identified 16 nonadherent mutants. All of these mutants lacked surface fibers and contained an insertion in the same open reading frame, which encodes a 157-kDa protein designated Cha for cryptic haemophilus adhesin. Analysis of the predicted amino acid sequence of Cha revealed the presence of an N-terminal signal peptide and a C-terminal domain bearing homology to YadA-like and Hia-like trimeric autotransporters. Examination of the C-terminal 120 amino acids of Cha demonstrated mobility as a trimer on sodium dodecyl sulfate-polyacrylamide gel electrophoresis a...Continue Reading

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Citations

Nov 26, 2010·Journal of Bacteriology·David G Thanassi
Mar 2, 2012·Emerging Infectious Diseases·Fiona R StroutsStephen D Bentley
Oct 3, 2009·FEMS Microbiology Letters·Michelle C Daigneault, Reggie Y C Lo
Oct 8, 2016·Molecular Oral Microbiology·J B KaplanN V Balashova

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