Identification of a novel type 1 diabetes-specific epitope by screening phage libraries with sera from pre-diabetic patients

Journal of Molecular Biology
C MennuniA Luzzago

Abstract

We used random peptide libraries displayed on phage to search for ligands to insulin dependent diabetes mellitus-related antibodies and were able to identify several candidate disease-related peptides. One of them, clone 92, showed a significant difference in the frequency of reactivity with the sera of patients and normal controls. Human immunoglobulins immunopurified on phage 92 specifically stained the islets on human pancreatic sections. When injected into rabbits, the selected peptide elicited antibodies that also stained human and rat pancreatic sections, with a pattern similar to that observed with immunoglobulins purified from the sera of patients. No reactivity was observed in other tissues. Our results indicate that the peptide identified in this work mimics a novel, diabetes-related self-antigen.

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J K ScottI J Goldstein
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K R OldenburgM A Gallop
Dec 1, 1991·The Journal of Clinical Endocrinology and Metabolism·L CastañoG S Eisenbarth
Sep 1, 1990·The Journal of Experimental Medicine·M R ChristieT L Delovitch
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J P Tam
Jun 1, 1981·The Journal of Experimental Medicine·T OnoderaA L Notkins
Jul 1, 1994·Trends in Biotechnology·R CorteseA Nicosia
Nov 1, 1994·European Journal of Immunology·R OrlandiF Felici
Feb 1, 1995·Current Opinion in Biotechnology·R CorteseM Sollazzo
Jan 1, 1993·Methods in Enzymology·G P Smith, J K Scott
Oct 1, 1993·The Journal of Clinical Investigation·M A Atkinson, N K Maclaren
Jan 1, 1996·Methods in Enzymology·F FeliciR Cortese
Jun 1, 1996·Journal of Autoimmunity·C MennuniA Luzzago
Jan 1, 1995·Biotechnology Annual Review·F FeliciC Traboni

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Citations

Nov 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·John T BallewPatrick S Daugherty
Nov 27, 1998·Nature Biotechnology·F BartoliP Monaci
Jul 5, 2013·PloS One·Xinyue LiuYurij Ionov
Apr 29, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·K ManoutcharianG Gevorkian

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