Identification of a Novel UT-B Urea Transporter in Human Urothelial Cancer

Frontiers in Physiology
Ruida HouGuangping Chen

Abstract

The urea transporter UT-B is widely expressed and has been studied in erythrocyte, kidney, brain and intestines. Interestingly, UT-B gene has been found more abundant in bladder than any other tissue. Recently, gene analyses demonstrate that SLC14A1 (UT-B) gene mutations are associated with bladder cancer, suggesting that urea transporter UT-B may play an important role in bladder carcinogenesis. In this study, we examined UT-B expression in bladder cancer with human primary bladder cancer tissues and cancer derived cell lines. Human UT-B has two isoforms. We found that normal bladder expresses long form of UT-B2 but was lost in 8 of 24 (33%) or significantly downregulated in 16 of 24 (67%) of primary bladder cancer patients. In contrast, the short form of UT-B1 lacking exon 3 was detected in 20 bladder cancer samples. Surprisingly, a 24-nt in-frame deletion in exon 4 in UT-B1 (UT-B1Δ24) was identified in 11 of 20 (55%) bladder tumors. This deletion caused a functional defect of UT-B1. Immunohistochemistry revealed that UT-B protein levels were significantly decreased in bladder cancers. Western blot analysis showed a weak UT-B band of 40 kDa in some tumors, consistent with UT-B1 gene expression detected by RT-PCR. Interestingl...Continue Reading

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Citations

Nov 18, 2019·Pflügers Archiv : European journal of physiology·Lanying YuYanwei Du
Dec 25, 2019·Physiological Reports·Alan Farrell, Gavin Stewart
Aug 14, 2018·Physiological Reports·Caragh WalpoleGavin Stewart
Mar 27, 2019·Journal of Cellular and Molecular Medicine·Marian MansoBahareh Vahabi

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Datasets Mentioned

BETA
KY706499

Methods Mentioned

BETA
PCR
electrophoresis
Protein Assay
pulldown
glycosylation
urine storage

Software Mentioned

iCycler
Invitrogen Primer

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