Abstract
Aspergillus fumigatus is a critical human fungal pathogen that infects the host via inhalation of airborne conidia. These conidia then germinate to form filamentous hyphae, which secrete various elements to survive in the host lung.Elements such as proteins secreted by A. fumigatus can act as virulence factors in host tissues. Among secreted proteins, we were interested in the thaumatin-like proteins of A. fumigatus. In our analysis of the function of thaumatin-like proteins, we found that, like CalA and CalB, CalC has a secreted form. Originally, CalC was predicted to be a GPI-anchored protein, as documented in the Aspergillus Genome Database. Here, we report on a novel secreted form of CalC. Furthermore, we established two novel hybridomas, C103 and C306, which recognized CalC. Monoclonal antibodies produced by these hybridomas responded to recombinant CalC produced by the mammalian cell line HEK293T and to the supernatant of cultured A. fumigatus.Taken together, our data suggest that calC can be spliced to give rise to a novel secretory form of CalC, which is present in the supernatant of cultured A. fumigatus. The hybridomas that we established will be helpful in understanding the biological role of A. fumigatus CalC.
References
Dec 15, 1991·Gene·H NiwaJ Miyazaki
Apr 1, 1994·Infection and Immunity·P SuttonA Müllbacher
Jan 1, 1995·Journal of Clinical Laboratory Analysis·K MitsutakeK Hara
Nov 7, 2001·The Journal of Membrane Biology·G GalliciottiR Klemenz
Nov 13, 2004·The Journal of Allergy and Clinical Immunology·Charles E Reed, Hirohito Kita
Aug 21, 2007·Fungal Genetics and Biology : FG & B·Ravit BelaishNir Osherov
May 12, 2009·Journal of Medical Microbiology·Santosh Kumar UpadhyayTaruna Madan
Dec 28, 2010·Journal of Pharmacological and Toxicological Methods·Shogo TakatsukaJoe Chiba
Jul 27, 2011·Medical Mycology Journal·Hikaru KumeMasahiko Okudaira
Nov 9, 2011·Nucleic Acids Research·Jason E StajichDavid S Roos
Jan 29, 2013·FEBS Letters·Akinori OkumuraSatoshi Yamagoe
Nov 7, 2013·Nucleic Acids Research·Gustavo C CerqueiraJennifer R Wortman
Apr 20, 2016·Frontiers in Microbiology·Carys A CroftScott J Tebbutt
May 18, 2016·BMC Genomics·Daisuke HagiwaraTohru Gonoi
Nov 15, 2016·Nature Microbiology·Hong LiuScott G Filler
Nov 8, 2017·Cellular Microbiology·Azusa Takahashi-NakaguchiTohru Gonoi
Nov 9, 2017·Cellular Microbiology·Shizhu ZhangDonald C Sheppard
Aug 25, 2018·The Journal of Biological Chemistry·Rajashri ShendeArvind Sahu
Feb 9, 2019·Journal of Applied Microbiology·I A RosaB M Heinzmann