Identification of a nuclear-localized nuclease from wheat cells undergoing programmed cell death that is able to trigger DNA fragmentation and apoptotic morphology on nuclei from human cells.

The Biochemical Journal
Fernando Domínguez, Francisco Javier Cejudo

Abstract

PCD (programmed cell death) in plants presents important morphological and biochemical differences compared with apoptosis in animal cells. This raises the question of whether PCD arose independently or from a common ancestor in plants and animals. In the present study we describe a cell-free system, using wheat grain nucellar cells undergoing PCD, to analyse nucleus dismantling, the final stage of PCD. We have identified a Ca2+/Mg2+ nuclease and a serine protease localized to the nucleus of dying nucellar cells. Nuclear extracts from nucellar cells undergoing PCD triggered DNA fragmentation and other apoptotic morphology in nuclei from different plant tissues. Inhibition of the serine protease did not affect DNA laddering. Furthermore, we show that the nuclear extracts from plant cells triggered DNA fragmentation and apoptotic morphology in nuclei from human cells. The inhibition of the nucleolytic activity with Zn2+ or EDTA blocked the morphological changes of the nucleus. Moreover, nuclear extracts from apoptotic human cells triggered DNA fragmentation and apoptotic morphology in nuclei from plant cells. These results show that degradation of the nucleus is morphologically and biochemically similar in plant and animal cells....Continue Reading

References

Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Jan 1, 1995·Methods in Cell Biology·J F KerrB V Harmon
Jun 1, 1995·Current Opinion in Cell Biology·W C Earnshaw
Dec 1, 1996·Plant Molecular Biology·M WangR A Schilperoort
May 20, 1998·Current Biology : CB·R HalenbeckL T Williams
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·X LiuX Wang
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Mar 31, 1999·The Plant Journal : for Cell and Molecular Biology·F X Xu, M L Chye
Apr 24, 1999·The Plant Cell·F Marty
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M SchmidC Gietl
May 4, 2000·Plant Molecular Biology·T E Young, D R Gallie
Oct 21, 2000·Genes & Development·V C Bromleigh, L P Freedman
Feb 24, 2001·Plant Molecular Biology·M SugiyamaH Fukuda
Jun 14, 2002·Experimental Cell Research·Hervé Lecoeur
Nov 1, 1996·Plant Physiology·F. Dominguez, F. J. Cejudo
Jul 1, 1997·The Plant Cell·R. I. Pennell, C. Lamb
Oct 24, 2002·Current Opinion in Plant Biology·Hideo Kuriyama, Hiroo Fukuda
Mar 26, 2003·Journal of Experimental Botany·Howard ThomasAnthony D Stead
Jun 6, 2003·The Plant Journal : for Cell and Molecular Biology·Janneke BalkPaul F McCabe
Nov 12, 2003·Planta·Annkatrin RoseIris Meier
Dec 31, 2003·The Journal of Biological Chemistry·Fernando DomínguezFrancisco Javier Cejudo
Jan 28, 2004·Cell·Nika N Danial, Stanley J Korsmeyer
Mar 17, 2004·Molecular and Cellular Biology·Francisco RomeroMaría Tortolero
Apr 9, 2004·Nature Reviews. Molecular Cell Biology·Eric Lam
May 4, 2004·Current Biology : CB·Maria F SuarezPeter V Bozhkov
Aug 7, 2004·Science·Noriyuki HatsugaiIkuko Hara-Nishimura
Nov 9, 2004·Current Biology : CB·Enrique RojoNatasha V Raikhel
Mar 8, 2005·Trends in Plant Science·Wouter G van Doorn, Ernst J Woltering
Mar 9, 2005·Trends in Cell Biology·Eric Lam
Mar 12, 2005·Plant Physiology·Maite SanmartínEnrique Rojo
Jun 9, 2005·Current Opinion in Plant Biology·Ikuko Hara-NishimuraMikio Nishimura
Jul 27, 2005·The Journal of Biological Chemistry·Miwa KuroyanagiIkuko Hara-Nishimura
Aug 17, 2005·Nature Reviews. Molecular Cell Biology·Kumiko Samejima, William C Earnshaw
Sep 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Peter V BozhkovAndrei Smertenko
Mar 1, 1997·Trends in Cell Biology

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Citations

Jul 9, 2013·Journal of Experimental Botany·María Paula López-Fernández, Sara Maldonado
Nov 22, 2013·BMC Plant Biology·Núria Sánchez-Pons, Carlos M Vicient
Nov 3, 2012·Plant Science : an International Journal of Experimental Plant Biology·Fernando Domínguez, Francisco Javier Cejudo
May 8, 2010·Journal of Plant Physiology·Krzysztof LeśniewiczElzbieta Poreba
Jun 15, 2012·Journal of Integrative Plant Biology·Hui-Min ChenXin-Qiang He
Aug 11, 2015·Plant Science : an International Journal of Experimental Plant Biology·María Paula López-FernándezSara Maldonado
Mar 28, 2012·Protein Expression and Purification·Kunyuan GuoXinxin Zhang
Aug 23, 2011·Plant Physiology and Biochemistry : PPB·Amangeldy K BissenbaevMurat K Saparbaev
Aug 15, 2014·Frontiers in Plant Science·Fernando Domínguez, Francisco J Cejudo
Dec 3, 2014·Trends in Plant Science·Tom Van HautegemMoritz K Nowack
Oct 30, 2016·Journal of Experimental Botany·D LatrasseM Delarue
Jun 15, 2016·Annual Review of Cell and Developmental Biology·Anna DanevaMoritz K Nowack
Mar 4, 2008·Journal of Experimental Botany·Wouter G van Doorn, Ernst J Woltering
Nov 23, 2018·Scientific Reports·María Paula López-FernándezSara Maldonado
May 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·A K M Mahmudul HuqueJeong Sheop Shin
Feb 22, 2021·Tree Physiology·Vera InácioLeonor Morais-Cecílio
Feb 2, 2022·Planta·G N Mohan KumarN Richard Knowles

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis