PMID: 2116014Aug 1, 1990Paper

Identification of a nucleotide exchange-promoting activity for p21ras

Proceedings of the National Academy of Sciences of the United States of America
J DownwardR A Weinberg

Abstract

The biological activity of proteins encoded by the ras family of oncogenes is dependent on whether they are bound to GTP or GDP: the type of nucleotide bound is dependent on the rate of GTP hydrolysis (promoted by the GTPase-activating protein, GAP) and the rate of nucleotide exchange with cytosolic pools. A protein that stimulates the rate of exchange of guanine nucleotide on p21ras has been identified and characterized in cytoplasmic extracts of human placenta. The exchange-promoting protein runs on a gel filtration column with an apparent relative molecular weight of about 60,000. It is sensitive to heat and to trypsin. The exchange-promoting protein acts reversibly and does not cause degradation of p21ras. It is inactive towards the alpha subunit of a heterotrimeric GTP-binding protein (Go alpha) but acts on a large number of different mutant ras proteins, including transforming and effector mutants that are insensitive to the action of GAP. This protein, which we have termed REP (ras exchange-promoting), has the characteristics expected of a physiological activator of p21ras in cellular growth-signal-transduction pathways.

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Citations

Mar 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Downward
Jan 1, 1993·Developmental Genetics·L ChenM F Moran
Mar 1, 1994·Cancer Metastasis Reviews·R Khosravi-Far, C J Der
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Aug 18, 2012·Nature Reviews. Cancer·Peter K Vogt
Jun 24, 2008·Nature Reviews. Molecular Cell Biology·Antoine E Karnoub, Robert A Weinberg
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A ValenciaC Sander
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T MizunoY Takai
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J E DeClueD R Lowy
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D BowtellP Senior
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·V DuronioJ W Schrader
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Ikawa, R A Weinberg
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Mar 1, 1992·Immunology Today·J DownwardD Cantrell
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Jun 2, 1992·Biochemistry·C K FoleyM W Anderson
Jun 5, 2003·Nature Reviews. Cancer·Marcos Malumbres, Mariano Barbacid

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