Identification of a palindromic sequence that is responsible for the up-regulation of NAPDH-ferredoxin reductase in a ferredoxin I deletion strain of Azotobacter vinelandii.

The Journal of Biological Chemistry
S M Yannone, B K Burgess

Abstract

Azotobacter vinelandii ferredoxin I (AvFdI) is one member of a class of 7Fe ferredoxins found in a variety of organisms that are all capable of aerobic growth. Disruption of the fdxA gene, which encodes AvFdI, leads to overexpression of its redox partner, NADPH-ferredoxin reductase (FPR). In this study the mechanism of FdI-mediated regulation of FPR was investigated. Northern analysis has shown that regulation is at the level of fpr transcription, the start site for transcription has been identified, and it is preceded by a canonical sigma 70-type bacterial promoter. Gel mobility shift assays show that there is a putative regulatory protein in A. vinelandii that binds specifically upstream of the -35 region. That protein is not AvFdI. A palindromic sequence was identified as a putative binding site, and randomization of that sequence completely eliminates binding of the putative regulatory protein. A luciferase reporter gene was placed under control of the A. vinelandii fpr promoter and introduced into wild type and FdI- strains of A. vinelandii. Luciferase activity was enhanced 7-fold in the FdI- mutant relative to the wild type. Alteration of the palindromic sequence reduced the luciferase levels in the FdI- strain to those o...Continue Reading

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D J HaileR D Klausner
Jul 11, 1990·Nucleic Acids Research·M L MichaelsJ H Miller
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P J StephensB K Burgess
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G H StoutL H Jensen
Apr 15, 1983·Archives of Biochemistry and Biophysics·H Beinert, A J Thomson
Aug 1, 1983·Archives of Biochemistry and Biophysics·G D Green, E Shaw
Sep 8, 1995·The Journal of Biological Chemistry·J M IsasB K Burgess
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N KhoroshilovaP J Kiley
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·J R LambertM G Cumsky
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·S I LiochevI Fridovich
Feb 1, 1994·Protein Expression and Purification·A VázquezB Burgess
Mar 1, 1993·Acta Crystallographica. Section D, Biological Crystallography·E A MerrittW H Orme-Johnson

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Citations

Dec 29, 1998·Protein Science : a Publication of the Protein Society·G Sridhar PrasadC D Stout
May 12, 2004·Biochimica Et Biophysica Acta·Eduardo A CeccarelliNéstor Carrillo
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·K RegnströmB K Burgess
Oct 9, 2015·Standards in Genomic Sciences·Pamela BhattacharyaSteven M Yannone
Dec 6, 2011·Current Opinion in Biotechnology·Steven M YannoneJohn A Tainer

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