Identification of a PDZ protein, PIST, as a binding partner for Rho effector Rhotekin: biochemical and cell-biological characterization of Rhotekin-PIST interaction.

The Biochemical Journal
Hidenori ItoKoh-ichi Nagata

Abstract

Among various effector proteins for the small GTPase Rho, the function(s) of Rhotekin is (are) almost unknown. We have identified PIST [PDZ (PSD-95, Discs-large and ZO-1) domain protein interacting specifically with TC10 (a Rho-family small GTPase)] as a binding partner for Rhotekin, using yeast two-hybrid screening. Rhotekin was found to associate with PIST in vitro and in both polarized and non-polarized MDCK (Madin-Darby canine kidney) cells. The C-terminal SPV (Ser-Pro-Val) motif of Rhotekin exhibited binding to the PDZ domain of PIST. The binding was markedly inhibited by an activated version of Rho and partially by that of Rac or Cdc42 in COS7 cells. In contrast, TC10 had no effects on the binding. Immunofluorescence analyses revealed the co-localization of PIST and Rhotekin at the Golgi apparatus in non-polarized fibroblast-like MDCK cells and AJs (adherens junctions) in the fully polarized cells. PIST and Rhotekin are recruited from the cytosol to AJs as the cell becomes polarized. Expression of constitutively active Rho or prevention of Rhotekin-PIST interaction induced diffuse cytoplasmic distribution of Rhotekin in polarized MDCK cells. These results suggest that there is (1) Rho-dependent regulation of Rhotekin-PIST...Continue Reading

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Citations

Mar 19, 2009·Medical Molecular Morphology·Koh-Ichi NagataTomiko Asano
Jan 10, 2012·The Journal of Biological Chemistry·Ganesh V PusapatiThomas Seufferlein
Dec 11, 2008·Science Signaling·Igor F TsigelnySanjay K Nigam
Sep 22, 2007·Trends in Pharmacological Sciences·Gunnar Schulte, Vítezslav Bryja
Sep 13, 2013·Small GTPases·Kathleen O'Connor, Min Chen
Jan 27, 2015·Cell and Tissue Research·Ruifeng LuJean M Wilson
May 11, 2019·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Wen Yuan LuoHong Zhang
Nov 18, 2008·Journal of Neurogenetics·Daisuke Yamamoto
Jul 25, 2018·International Journal of Molecular Sciences·Hidenori ItoKoh-Ichi Nagata

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