Identification of a peptide for folate receptor alpha by phage display and its tumor targeting activity in ovary cancer xenograft

Scientific Reports
Lijun XingMin Wang

Abstract

The expression level of folate receptor alpha (FRα) is located highly rate in ovarian cancer though it is remained absent in normal tissues. This highly tumor restricted expression profile makes FRα a promising target for tumor therapy and diagnosis. In this research we report a FRα binding peptide C7(Met-His-Thr-Ala-Pro-Gly-Trp-Gly-Tyr-Arg-Leu-Ser) discovered by phage display and this peptide showed specific binding to FRα expressing cells by cell ELISA and flow cytometry. Tumor targeting ability of C7 was proved in vivo by both phage homing experiment and fluorescence imaging. C7 can be internalized by SKOV3 cells and its affinity to FRα was determined by MST. The molecular recognition was revealed by structure modeling, suggesting its binding mode with FRα.

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H MatsueS W Lacey
Jan 1, 1990·Annual Review of Nutrition·G B Henderson
Jul 3, 1998·The EMBO Journal·M LauwereysS Muyldermans
Feb 15, 2001·The Journal of Thoracic and Cardiovascular Surgery·R BuenoD Sugarbaker
Mar 26, 2003·Journal of Microbiological Methods·Valery A Petrenko, Vitaly J Vodyanoy
Apr 20, 2004·Advanced Drug Delivery Reviews·Christopher P Leamon, Joseph A Reddy
Jun 25, 2004·Nucleic Acids Research·Stephen R ComeauCarlos J Camacho
Feb 3, 2006·International Journal of Cancer. Journal International Du Cancer·Linda E Kelemen
Aug 26, 2006·Proteins·Dima KozakovSandor Vajda
May 10, 2007·International Journal of Cancer. Journal International Du Cancer·Lynn C HartmannPhilip S Low
Jul 21, 2007·Proteomics·Tord BerggårdPeter James
Sep 19, 2007·Molecular Pharmaceutics·Christopher P LeamonYu Wang
Nov 3, 2009·Drug Discovery Today·Patrick VliegheMichel Khrestchatisky
Apr 30, 2013·Biotechnology Advances·Janka BábíčkováPeter Celec
Nov 21, 2015·Journal of Chemical Theory and Computation·Yimin ShenPierre Tufféry
Jun 2, 2016·Oncotarget·Anthony CheungSophia N Karagiannis
Jan 13, 2017·Nature Protocols·Dima KozakovSandor Vajda

❮ Previous
Next ❯

Citations

May 6, 2019·International Journal of Molecular Sciences·Roopa DharmattiYoshihiro Ito
Sep 10, 2020·International Journal of Peptide Research and Therapeutics·Chunyan LiHong Yang
May 30, 2019·Protein & Cell·Phei Er Saw, Er-Wei Song
Aug 23, 2020·International Journal of Molecular Sciences·Thomas A DavidsonDavid H Kohn
Jan 23, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Armin AhmadiKyung-Ho Roh
Feb 22, 2021·Journal of Neuro-oncology·Nikita LakomkinConstantinos G Hadjipanayis
Jul 17, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yefei YuDu Cheng

❮ Previous
Next ❯

Methods Mentioned

BETA
phage display
ELISA
ELISAs
flow cytometry
flow
xenografts
Fluorescence Imaging
confocal microscopy
Transfection
Microscale Thermophoresis

Software Mentioned

Pymol
ClusPro
GraphPad
Pepfold3
GraphPad Prism
MOE
NT
GROMACS

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Journal of Peptide Science : an Official Publication of the European Peptide Society
Chuying MaZhongbing Huang
Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
A KoivistoinenJ A Salo
Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine
Wu XingLi Ao
© 2022 Meta ULC. All rights reserved