Identification of a polymorphic gene, BCL2A1, encoding two novel hematopoietic lineage-specific minor histocompatibility antigens

The Journal of Experimental Medicine
Yoshiki AkatsukaToshitada Takahashi

Abstract

We report the identification of two novel minor histocompatibility antigens (mHAgs), encoded by two separate single nucleotide polymorphisms on a single gene, BCL2A1, and restricted by human histocompatibility leukocyte antigen (HLA)-A*2402 (the most common HLA-A allele in Japanese) and B*4403, respectively. Two cytotoxic T lymphocyte (CTL) clones specific for these mHAgs were first isolated from two distinct recipients after hematopoietic cell transplantation. Both clones lyse only normal and malignant cells within the hematopoietic lineage. To localize the gene encoding the mHAgs, two-point linkage analysis was performed on the CTL lytic patterns of restricting HLA-transfected B lymphoblastoid cell lines obtained from Centre d'Etude du Polymorphisme Humain. Both CTL clones showed a completely identical lytic pattern for 4 pedigrees and the gene was localized within a 3.6-cM interval of 15q24.3-25.1 region that encodes at least 46 genes. Of those, only BCL2A1 has been reported to be expressed in hematopoietic cells and possess three nonsynonymous nucleotide changes. Minigene transfection and epitope reconstitution assays with synthetic peptides identified both HLA-A*2402- and B*4403-restricted mHAg epitopes to be encoded by di...Continue Reading

References

Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M LathropJ Ott
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·P G CoulieT Boon
Jul 1, 1994·Human Heredity·A A SchäfferR W Cottingham
Oct 1, 1993·Current Opinion in Immunology·J L Ferrara
Nov 22, 1997·Current Opinion in Immunology·E Simpson, D Roopenian
Aug 27, 1998·American Journal of Human Genetics·K W BromanJ L Weber
Jan 20, 1999·The Journal of Experimental Medicine·H DolstraE van de Wiel-van Kemenade
Dec 22, 1999·Immunogenetics·H RammenseeS Stevanović
Feb 29, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E H WarrenS R Riddell
Apr 25, 2000·Journal of Molecular Biology·C KuttlerK P Hadeler
Jun 13, 2000·The Journal of Biological Chemistry·P J DuriezA Karsan
Jan 11, 2000·Nucleic Acids Research·D L WheelerB A Rapp
Jan 10, 2001·The Journal of Experimental Medicine·A G BricknerS R Riddell
Sep 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R A PierceV H Engelhard
May 2, 2002·Protein Engineering·Can KeşmirSøren Brunak
Jun 5, 2002·Genome Research·W James KentDavid Haussler
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bregje MommaasTuna Mutis
Oct 2, 2002·European Journal of Immunology·Harry DolstraElly van de Wiel-van Kemenade

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Citations

Dec 13, 2005·Immunogenetics·Mathias M SchulerStefan Stevanovic
Jan 1, 2010·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Elizabeth Ingulli
Mar 16, 2005·Current Opinion in Immunology·Lothar Hambach, Els Goulmy
Apr 23, 2004·Trends in Immunology·Eric SpieringsEls Goulmy
Sep 10, 2003·Experimental Hematology·J H Frederik FalkenburgRoel Willemze
Feb 9, 2011·Immunology and Cell Biology·Marie Bleakley, Stanley R Riddell
Mar 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Marieke GriffioenJ H Frederik Falkenburg
Aug 14, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Scott S TykodiEdus H Warren
Feb 18, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yishai OfranJerome Ritz
Dec 3, 2005·The Journal of Clinical Investigation·Eric Spierings, Els Goulmy
Sep 24, 2008·Blood·Michi KameiYoshiki Akatsuka
Apr 10, 2014·Nature Communications·Diana Paola GranadosClaude Perreault
Nov 19, 2015·Molecular Oncology·J H F Falkenburg, I Jedema
Apr 24, 2007·Best Practice & Research. Clinical Haematology·Lothar HambachEls Goulmy
Dec 21, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A HeinoldT H Tran
Jun 8, 2006·Vox Sanguinis·L D Barber, J A Madrigal
Nov 4, 2008·International Journal of Immunogenetics·E Spierings
May 25, 2007·Cancer Science·Yoshiki AkatsukaToshitada Takahashi
Sep 19, 2006·British Journal of Haematology·Yoshiki AkatsukaToshitada Takahashi
Aug 9, 2006·International Journal of Immunogenetics·M Halling-BrownD S Moss

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Methods Mentioned

BETA
FCS
PCR
antisense oligonucleotides
genotyping
dot blot

Software Mentioned

PAPROC
SYFPEITHI
Map Viewer
GeneScan
SAGEmap
BIMAS
UCSC genome browser
MLINK
NetChop
FASTLINK

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