Identification of a post-transcriptional regulatory element in the human endogenous retroviral syncytin-1

The Journal of General Virology
Koichi KitaoTakayuki Miyazawa

Abstract

Retroviral transcripts have cis-acting elements that interact with host and viral proteins to enable efficient nuclear export and/or translation; however, it is poorly understood whether the transcripts of human endogenous retroviral genes retain such elements. Here, we show that human syncytin-1, which is derived from human endogenous retrovirus W, requires a 3' untranslated region (3'UTR) for efficient gene expression and retains a post-transcriptional regulatory element (named SPRE). The insertion of SPRE markedly increased a reporter gene (human immunodeficiency virus type 1 Gag) expression without affecting the amounts of nuclear or cytoplasmic transcript. Deletion analysis identified a required sequence for SPRE activity, and the prediction of the RNA secondary structure demonstrated a common secondary structure found among active SPRE sequences. Another human syncytin, syncytin-2, also requires a 3'UTR for efficient gene expression. These data provide insights into post-transcriptional regulation in endogenous retroviral gene expression.

References

Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·M BrayM L Hammarskjöld
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Oct 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sandra BlaiseThierry Heidmann
Jan 1, 2004·Genes & Development·Li JinMarie-Louise Hammarskjöld
Feb 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·François MalletBernard Mandrand
Apr 14, 2004·Journal of Virology·Rozanne M Sandri-Goldin
Jan 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Anne DupressoirThierry Heidmann
Mar 26, 2011·PLoS Genetics·Polina PerelmanJill Pecon-Slattery
Aug 9, 2011·Nature Structural & Molecular Biology·Marianna TeplovaDinshaw J Patel
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Guillaume CornelisThierry Heidmann
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Guillaume CornelisAnne Dupressoir
Jan 31, 2014·Journal of Virology·Toshie SakumaYasuhiro Ikeda
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Guillaume CornelisThierry Heidmann
Jan 29, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Joachim Denner

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