PMID: 9442103Feb 28, 1998Paper

Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc6, but dependent on cdk2

The Journal of Cell Biology
X H Hua, J Newport

Abstract

Before initiation of DNA replication, origin recognition complex (ORC) proteins, cdc6, and minichromosome maintenance (MCM) proteins bind to chromatin sequentially and form preinitiation complexes. Using Xenopus laevis egg extracts, we find that after the formation of these complexes and before initiation of DNA replication, cdc6 is rapidly removed from chromatin, possibly degraded by a cdk2-activated, ubiquitin-dependent proteolytic pathway. If this displacement is inhibited, DNA replication fails to initiate. We also find that after assembly of MCM proteins into preinitiation complexes, removal of the ORC from DNA does not block the subsequent initiation of replication. Importantly, under conditions in which both ORC and cdc6 protein are absent from preinitiation complexes, DNA replication is still dependent on cdk2 activity. Therefore, the final steps in the process leading to initiation of DNA replication during S phase of the cell cycle are independent of ORC and cdc6 proteins, but dependent on cdk2 activity.

References

Jul 1, 1992·Molecular and Cellular Biology·S KornbluthJ W Newport
May 1, 1991·The Journal of Cell Biology·L M RoyJ L Maller
Jun 1, 1991·Genes & Development·K M HennessyD Botstein
Apr 15, 1974·Journal of Molecular Biology·L M Hereford, L H Hartwell
Dec 8, 1995·Science·K A GavinB Stillman
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Dalton, L Whitbread
Jun 1, 1995·The Journal of Cell Biology·I T TodorovS E Kearsey
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·H Rao, B Stillman
Feb 1, 1993·Molecular Biology of the Cell·H J YoonJ L Campbell
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·M Muzi FalconiT J Kelly
Jun 14, 1996·Cell·J Wuarin, P Nurse
Apr 1, 1996·Current Opinion in Genetics & Development·S Donovan, J F Diffley
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·M HendricksonJ Gautier
Feb 14, 1997·Science·J Walter, J W Newport

❮ Previous
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Citations

Mar 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M L DePamphilis
Jun 27, 2000·Journal of Cellular Physiology·J A BoganM L Depamphilis
Mar 10, 2001·Journal of Cellular Physiology·P R Yew
Feb 22, 2002·Journal of Cellular Physiology·Hisao Masai, Ken-Ichi Arai
Nov 8, 2011·Chromosoma·Angelos Constantinou
Mar 31, 2004·Experimental Cell Research·Domenico MaioranoMarcel Méchali
Aug 19, 2003·Developmental Biology·Nadia Tikhmyanova, Thomas R Coleman
Aug 14, 2003·Experimental Cell Research·David T MahaffeyMartin Rechsteiner
Nov 7, 2000·Current Opinion in Cell Biology·H TakisawaY Kubota
Jun 26, 1998·Current Opinion in Cell Biology·P Pasero, S M Gasser
Jan 23, 1999·Current Opinion in Cell Biology·J Leatherwood
Mar 11, 1999·Current Opinion in Genetics & Development·A D Donaldson, J J Blow
Feb 19, 2002·Trends in Cell Biology·J Julian Blow, Ben Hodgson
Mar 12, 2003·Trends in Cell Biology·Cristina Pelizon
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·J Julian Blow, Anindya Dutta
Jun 13, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hideo Nishitani, Zoi Lygerou
Aug 9, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yoshihiko KonoNobuo Sakaguchi
Jan 25, 2000·The Journal of Biological Chemistry·T A Prokhorova, J J Blow
Mar 22, 2002·Molecular Biology of the Cell·Mark JackmanJonathon Pines
Apr 16, 2002·Molecular Biology of the Cell·Natalya S FrolovaThomas R Coleman
Oct 8, 1999·Molecular Biology of the Cell·S ElsasserJ L Campbell
Aug 6, 1999·Molecular Biology of the Cell·U HerbigE Fanning
Mar 11, 2010·Applied Immunohistochemistry & Molecular Morphology : AIMM·Lukasz KuźbickiBarbara W Chwirot
Dec 28, 2002·Genes & Development·Kenji ShimadaSusan M Gasser
Apr 25, 2000·Molecular and Cellular Biology·E Santoni-RugiuJ Lukas
Apr 25, 2000·Molecular and Cellular Biology·I PereverzevaM Coué
Oct 12, 2000·Molecular and Cellular Biology·H A SnaithS L Forsburg
Jan 3, 2001·Molecular and Cellular Biology·H LamanN Jones

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