PMID: 2498879Jun 1, 1989Paper

Identification of a protein associated with p21ras by chemical crosslinking

Proceedings of the National Academy of Sciences of the United States of America
J de GunzburgR A Weinberg

Abstract

The products of the ras oncogenes (p21ras) are ubiquitous membrane-associated proteins that bind guanine nucleotides and possess an intrinsic GTPase activity. Because of their functional homologies with regulatory guanine nucleotide-binding proteins, they are thought to be involved in the control of cellular proliferation as transducers of incoming growth signals. In an effort to identify proteins interacting with p21ras, we have used in vivo crosslinking techniques on Rat-1 fibroblasts and derived cell lines overexpressing p21ras and immunoprecipitation with polyclonal anti-p21ras antibodies. Under those conditions, using the homobifunctional crosslinker dithiobis(succinimidyl propionate), a protein of Mr 60,000 (p60) is found to be associated with p21ras, and this association is enhanced by the treatment of quiescent cells with serum. Upon sedimentation of detergent extracts from crosslinked cells on sucrose gradients, a p21-p60 complex could be demonstrated with a Mr of 200,000-300,000, p60 does not appear to be related to pp60src nor to the cytosolic GTPase activating protein that interacts with p21ras to enhance its GTPase activity. The amount of p60 seems to be limiting relative to p21ras in fibroblasts, since similar lev...Continue Reading

References

Aug 12, 1988·Science·A Levitzki
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J DownwardR A Weinberg
Aug 20, 1987·Nature·M R Hanley, T Jackson
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J B GibbsE M Scolnick
Jan 1, 1987·Annual Review of Biochemistry·M Barbacid
Jul 14, 1987·Biochemistry·R A Roth, S B Pierce

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Citations

Apr 26, 1995·Molecular and Cellular Biochemistry·T K Chataway, G J Barritt
Mar 23, 1995·Molecular and Cellular Biochemistry·T K Chataway, G J Barritt
Mar 19, 2002·Molecular and Biochemical Parasitology·Maarten HoekGeorge A M Cross
Dec 2, 2000·Cell Proliferation·K HelmbrechtL Rensing
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G LeeI B Weinstein
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F BérangerJ de Gunzburg
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Ikawa, R A Weinberg
May 1, 1994·The Journal of Cell Biology·L ShumR Derynck
Jan 27, 2004·Infection and Immunity·Jennifer L WamplerArun K Bhunia
May 1, 1994·Cancer Causes & Control : CCC·I De VivoP W Brandt-Rauf
Aug 16, 1990·Biochemical and Biophysical Research Communications·G P Rossini, C Malaguti
Apr 8, 2017·Proteomes·Matthew P PadulaSteven P Djordjevic
Apr 29, 1992·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M R Webb
Jan 12, 2002·The Journal of Biological Chemistry·Vanessa NancyJean de Gunzburg
Oct 25, 1996·The Journal of Biological Chemistry·C GambyL Baizer
Mar 20, 1999·The Journal of Biological Chemistry·V NancyJ de Gunzburg
Sep 30, 1991·International Journal of Cancer. Journal International Du Cancer·B JimenezJ de Gunzburg
Jun 2, 2018·Methods : a Companion to Methods in Enzymology·Feixia ChuHieu T Nguyen
Mar 1, 1995·Microbiological Reviews·E M Phizicky, S Fields
Feb 1, 1994·Molecular and Cellular Biology·S MaheswaranG E Sonenshein

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