Identification of a protein isolated from senescent human cells that binds to hepatitis B virus X antigen

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
B S SunMark A Feitelson

Abstract

Hepatitis B virus-encoded X antigen contributes to the development of hepatocellular carcinoma. Given that X antigen functions by binding to other proteins, additional X-binding proteins were sought from an adult human liver cDNA library in a yeast two-hybrid system. The results yielded a clone encoding a 55-kd protein that is associated with replicative senescence (p55sen). Binding of p55sen to X antigen was confirmed in vitro by immunoprecipitation and affinity chromatography. The expression of endogenous p55sen inversely correlated with cell growth. Transient transfection of X antigen or p55sen into HepG2 cells stimulated DNA synthesis by twofold to threefold, whereas cotransfection did not, suggesting that these molecules functionally interact. The detection of p55sen in embryonic mouse liver, its absence in adult mouse and human livers, and its reappearance in livers from carriers with chronic liver disease, suggest that it may play important roles in the regulation of liver cell growth. The similarity between p55sen and a notch ligand, which is involved in cell fate determinations during embryogenesis, implies that the binding of p55sen by X antigen may also contribute to an alteration in cell fate, which is characteristi...Continue Reading

References

Jul 1, 1992·Clinical Microbiology Reviews·M Feitelson
Jul 1, 1992·Journal of Hepatology·I D DiamantisL Bianchi
Jul 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W L WangM A Feitelson
Jan 1, 1991·Journal of Hepatology·M SeiferW H Gerlich
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S Takada, K Koike
Jul 1, 1990·Virology·M A Feitelson, M M Clayton
Jul 1, 1989·Japanese Journal of Cancer Research : Gann·Y ShirakataK Koike
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M A SellsG Acs
Jan 1, 1981·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D A Shafritz, M C Kew
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·J Benn, R J Schneider
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·P ZagourasS Artavanis-Tsakonas
Apr 14, 1995·Science·S Artavanis-TsakonasM E Fortini
Feb 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P PaterliniC Brechot
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S R GrossmanE Kieff
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·X W WangC C Harris
Jul 1, 1996·Trends in Biochemical Sciences·M Rechsteiner, S W Rogers
May 1, 1996·Journal of Viral Hepatitis·F Henkler F, R Koshy
Jan 1, 1992·Methods in Molecular Biology·O Danos

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Citations

Sep 25, 1999·Journal of Cellular Physiology·M A Feitelson
Apr 13, 2012·PloS One·Ke YuanMark A Feitelson
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·J Guilherme TralhaoChristian Bréchot
Jun 26, 2002·Neoplasia : an International Journal for Oncology Research·N Lale Satiroglu TufanMark A Feitelson
Dec 26, 2006·Cancer Letters·Mark A Feitelson, Jungmin Lee
Oct 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hee Jeong KongJaeHun Cheong
May 13, 2003·Reviews in Medical Virology·Nir ParanYosef Shaul
Jun 26, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jiejie XuJianxin Gu
Apr 6, 2004·The Surgical Clinics of North America·Mark A FeitelsonZhaorui Lian
Jul 23, 2003·Biochimica Et Biophysica Acta·Arik CooperYosef Shaul
Aug 11, 2000·Seminars in Cancer Biology·C BréchotP Paterlini-Bréchot
Jan 17, 2015·Oncoscience·Yuanjie HuYi-Hong Zhou
Jan 14, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Hao ShenMin Zhu

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