Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens

PloS One
Yi WangChangtian Li

Abstract

Usnic acid is a unique polyketide produced by lichens. To characterize usnic acid biosynthesis, the transcriptome of the usnic-acid-producing lichen-forming fungus Nephromopsis pallescens was sequenced using Illumina NextSeq technology. Seven complete non-reducing polyketide synthase genes and nine highly-reducing polyketide synthase genes were obtained through transcriptome analysis. Gene expression results obtained by qPCR and usnic acid detection with LCMS-IT-TOF showed that Nppks7 is probably involved in usnic acid biosynthesis in N. pallescens. Nppks7 is a non-reducing polyketide synthase with a MeT domain that also possesses beta-ketoacyl-ACP synthase, acyl transferase, product template, acyl carrier protein, C-methyltransferase, and Claisen cyclase domains. Phylogenetic analysis shows that Nppks7and other polyketide synthases from lichens form a unique monophyletic clade. Taken together, our data indicate that Nppks7 is a novel PKS in N. pallescens that is likely involved in usnic acid biosynthesis.

References

Aug 22, 2001·Trends in Biotechnology·V MiaoJ Davies
Jun 14, 2002·Die Naturwissenschaften·Moreno CocchiettoGianni Sava
Nov 28, 2002·Phytochemistry·K Ingólfsdóttir
Oct 27, 2004·Antimicrobial Agents and Chemotherapy·I FrancoliniP Stoodley
Aug 13, 2005·Anti-cancer Drugs·Margareth MayerVirginia C L Appleyard
Sep 30, 2008·Mycological Research·Georg BrunauerMartin Grube
Nov 27, 2008·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Lei GuoVasilios H Frankos
Dec 23, 2008·Mycological Research·Andrey N GagunashviliOlafur S Andrésson
Dec 30, 2008·Journal of Bioscience and Bioengineering·Kanae SakaiTakuya Nihira
Apr 8, 2009·Bioorganic & Medicinal Chemistry Letters·Drew J HawranikJohn L Sorensen
Feb 23, 2010·Molecular BioSystems·James F SanchezClay C C Wang
Dec 31, 2010·Nature Reviews. Genetics·Fatih Ozsolak, Patrice M Milos
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Jan 31, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M BačkorováP Fedoročko
Apr 9, 2013·Annual Review of Analytical Chemistry·Elaine R Mardis
Apr 30, 2013·Journal of the American Chemical Society·Yi-Ming ChiangBerl R Oakley
Apr 26, 2014·Current Opinion in Biotechnology·Eric J N HelfrichJörn Piel
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura

❮ Previous
Next ❯

Citations

May 29, 2019·The New Phytologist·Veronika KuhnWolfgang Eisenreich
Mar 13, 2020·Genome Biology and Evolution·Bastian Greshake TzovarasIngo Ebersberger
Jan 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anjuli CalcheraImke Schmitt
Sep 13, 2018·Journal of Industrial Microbiology & Biotechnology·Robert L Bertrand, John L Sorensen
Aug 26, 2021·Metabolic Engineering Communications·James T KealeyPhilip J Barr

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP091413

Methods Mentioned

BETA
gene knockout
RNA-Seq
electrophoresis
PCR

Software Mentioned

BLASTX
SAT
Chrysalis
Clustal W
BLAST
Beacon Designer
Inchworm
MEGA
Butterfly
FGENESH

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.