Identification of a rat oltipraz-inducible UDP-glucuronosyltransferase (UGT1A7) with activity towards benzo(a)pyrene-7,8-dihydrodiol.

The Journal of Biological Chemistry
A D GroveJ K Ritter

Abstract

Previous work has shown that polycyclic aromatic hydrocarbons and oltipraz both induce an unidentified rat liver UDP-glucuronosyltransferase with activity toward benzo(a)pyrene-7, 8-diol, the proximate carcinogenic form of benzo(a)pyrene. Here we report the isolation of a benzo(a)pyrene-7,8-diol transferase-encoding cDNA, LC14, from an adult rat hepatocyte-derived cell line (RALA255-10G LCS-3). The predicted amino acid sequence of LC14 is nearly identical (5 differences out of 531 residues) to that deduced from UGT1A7, recently cloned at the genomic DNA level (Emi, Y., Ikushiro, S., and Kyanagi, T. (1995) J. Biochem. (Tokyo) 117, 392-399). Northern analysis of RNA from female F344 rat liver and LCS-3 cells revealed over a 40-fold and 4.4-fold enhancement by oltipraz treatment, respectively. Benzo(a)pyrene-7, 8-diol glucuronidating activity was detected (0.4 nmol/10(6) cells/16 h) in AHH-1 cells transfected with the LC14 expression vector, pMF6-LC14-3. The LC14-encoded transferase exhibited even higher activity toward certain benzo(a)pyrene phenols, including the major 3- and 9-phenol metabolites (4.1 and 2.8 nmol/10(6) cells/16 h, respectively). The Km of the enzyme for (-)-trans benzo(a)pyrene-7, 8-diol and 3-OH-BP was 15.5 an...Continue Reading

References

Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·K W Bock
Aug 1, 1990·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·D K MonteithS C Strom
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D HardingB Burchell
Jan 1, 1987·Archives of Toxicology·K W BockB S Bock-Henning
May 1, 1981·The Journal of Cell Biology·J Y Chou, S E Schlegel-Haueter
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Feb 15, 1980·Biochemical Pharmacology·K W BockK L Platt
Oct 1, 1993·Chemico-biological Interactions·T M Penning

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Citations

Jan 16, 1999·Journal of Biochemical and Molecular Toxicology·B A Carr, M R Franklin
Sep 21, 2002·Journal of Biochemical and Molecular Toxicology·John G Lamb, Michael R Franklin
Nov 15, 2011·Archives of Toxicology·Mohammad Monir TawfeeqKunitoshi Mitsumori
Aug 30, 2003·Toxicology and Applied Pharmacology·Diana J AuyeungJoseph K Ritter
Sep 20, 2001·Journal of the National Cancer Institute·Z ZhengP Lazarus
May 21, 1999·Drug Metabolism Reviews·K W BockB S Bock-Hennig
Oct 13, 2009·Drug Metabolism Reviews·Yuji IshiiHideyuki Yamada
Sep 22, 2010·Biochemical Pharmacology·M Cristina de AlmagroCarlos J Ciudad
Mar 21, 2006·Toxicology and Applied Pharmacology·Cheng-Ming LeeYi-Ming Arthur Chen
Mar 21, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Nobumitsu HaniokaShizuo Narimatsu
May 1, 2006·Environmental Toxicology and Pharmacology·Laura WebbJoseph K Ritter
Aug 1, 2012·Toxicology Letters·Daniela GradinaruJean-Marie Heydel
Apr 26, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ji-Youn HanJin Soo Lee
Jun 20, 2003·The Pharmacogenomics Journal·C Guillemette
Jan 17, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Laurence AntonioJacques Magdalou
Feb 21, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Fay K KesslerJoseph K Ritter
Oct 27, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Laura J WebbJoseph K Ritter
Feb 14, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·M K ShelbyC D Klaassen
Jun 28, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yuki TochigiHiroshi Yokota
Mar 20, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Zhong ZhengPhilip Lazarus
Feb 24, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Peter G WellsJoseph K Ritter
Feb 21, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Nichole R Vansell, Curtis D Klaassen
Jul 21, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·M K Shelby, C D Klaassen
Apr 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yue WangMasao Omata
Jul 2, 1998·Biochemical and Biophysical Research Communications·B Mojarrabi, P I Mackenzie
May 7, 2002·Biochemical Pharmacology·Yuet Kin Leung, John W Ho
Sep 21, 2013·Chemical Research in Toxicology·Li ZhangTrevor M Penning

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